Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
14
|
| pubmed:dateCreated |
1995-3-16
|
| pubmed:abstractText |
Influenza A/PR/8/34-derived chimeric (D) protein (SK&F 106160) composed of the first 81 amino acids (aa) of NS1 fused to the conserved 157 C-terminal aa of HA2 (NS1 1-81-HA2 65-222) was previously shown to induce H-2d-restricted protective cytotoxic T-lymphocyte (CTL) immunity in inbred mice. However, D protein, like other small peptides, exhibited haplotype dependence and was not immunogenic in H-2b and H-2K mice. A potential use of this antigen in humans and the role of T cells in any protection were evaluated in outbred Swiss and inbred CBF6F1 (H-2d/b) mice. Mice immunized with D protein and challenged by small-particle aerosol with a lethal dose of influenza virus were significantly protected against mortality from influenza A/H1N1 and A/H2N2 (p < 0.05-< 0.0000001), but not from A/H3N2 and influenza B viruses when compared with control mice. D protein did not induce serum virus-neutralizing antibody but caused virus to be cleared faster in immunized mice. Protection was long-lasting. In vivo depletion of either Lyt2 (CD8+) or L3T4 (CD4+) T cells with monoclonal antibodies led to abrogation of in vitro-generated CTL activity in CF6F1 mice and significant reduction in the protective efficacy of D protein against virus challenge in both Swiss and CF6F1 mice. These results suggest that protection was mediated by CD8+ and/or CD4+ cells and not antibody. Thus D protein, via a conserved sequence on the HA2 polypeptide, has the potential to induce partially cross-reactive CTL that may protect against influenza virus disease in humans.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aerosols,
http://linkedlifedata.com/resource/pubmed/chemical/D protein, influenza A...,
http://linkedlifedata.com/resource/pubmed/chemical/Influenza Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0264-410X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1340-8
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7856302-Aerosols,
pubmed-meshheading:7856302-Animals,
pubmed-meshheading:7856302-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7856302-CD8-Positive T-Lymphocytes,
pubmed-meshheading:7856302-Cross Reactions,
pubmed-meshheading:7856302-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:7856302-Female,
pubmed-meshheading:7856302-Flow Cytometry,
pubmed-meshheading:7856302-Influenza A virus,
pubmed-meshheading:7856302-Influenza Vaccines,
pubmed-meshheading:7856302-Mice,
pubmed-meshheading:7856302-Mice, Inbred BALB C,
pubmed-meshheading:7856302-Mice, Inbred C57BL,
pubmed-meshheading:7856302-Neutralization Tests,
pubmed-meshheading:7856302-Orthomyxoviridae Infections,
pubmed-meshheading:7856302-Recombinant Proteins,
pubmed-meshheading:7856302-Vaccines, Synthetic,
pubmed-meshheading:7856302-Viral Proteins,
pubmed-meshheading:7856302-Virus Replication
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Influenza A subtype cross-protection after immunization of outbred mice with a purified chimeric NS1/HA2 influenza virus protein.
|
| pubmed:affiliation |
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, TX 77030-3498.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|