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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1995-3-15
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pubmed:abstractText |
Distinct NF-kappa B subunit combinations contribute to the specificity of NF-kappa B-mediated transcriptional activation and to the induction of multiple cytokine genes including interferon-beta (IFN-beta). To evaluate the regulatory influence of different homo- and heterodimers, NF-kappa B subunits were analyzed for transcriptional activity in vitro using test templates containing two types of NF-kappa B recognition elements (the human immunodeficiency virus type 1 enhancer and the IFN-beta-positive regulatory domain-II (PRDII) as well as IFN-beta PRDIII-PRDI-PRDII linked to the -56 minimal promoter of rabbit beta-globin. Recombinant NF-kappa B subunits (p50, p65, c-Rel, p52, and I kappa B alpha) and interferon regulatory factor 1 were produced from either Escherichia coli or baculovirus expression systems. Transcriptional analysis in vitro demonstrated that 1) various dimeric complexes of NF-kappa B differentially stimulated transcription through the human immunodeficiency virus enhancer or PRDII up to 20-fold; 2) recombinant I kappa B alpha specifically inhibited NF-kappa B-dependent transcription in vitro; and 3) different NF-kappa B complexes and interferon regulatory factor 1 cooperated to stimulate transcription in vitro through the PRDIII-PRDI-PRDII virus-inducible regulatory domains of the IFN-beta promoter. These results demonstrate the role of NF-kappa B protein dimerization in differential transcriptional activation in vitro and emphasize the role of cooperativity between transcription factor families as an additional regulatory level to maintain transcriptional specificity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Globins,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-beta,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-rel,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3123-31
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7852394-Animals,
pubmed-meshheading:7852394-Baculoviridae,
pubmed-meshheading:7852394-Base Sequence,
pubmed-meshheading:7852394-Cloning, Molecular,
pubmed-meshheading:7852394-Cytokines,
pubmed-meshheading:7852394-DNA Primers,
pubmed-meshheading:7852394-DNA-Binding Proteins,
pubmed-meshheading:7852394-Escherichia coli,
pubmed-meshheading:7852394-Gene Expression,
pubmed-meshheading:7852394-Gene Expression Regulation,
pubmed-meshheading:7852394-Globins,
pubmed-meshheading:7852394-HIV Enhancer,
pubmed-meshheading:7852394-HIV-1,
pubmed-meshheading:7852394-I-kappa B Proteins,
pubmed-meshheading:7852394-Interferon-beta,
pubmed-meshheading:7852394-Molecular Sequence Data,
pubmed-meshheading:7852394-NF-kappa B,
pubmed-meshheading:7852394-Oligonucleotide Probes,
pubmed-meshheading:7852394-Promoter Regions, Genetic,
pubmed-meshheading:7852394-Proto-Oncogene Proteins,
pubmed-meshheading:7852394-Proto-Oncogene Proteins c-rel,
pubmed-meshheading:7852394-Rabbits,
pubmed-meshheading:7852394-Recombinant Proteins,
pubmed-meshheading:7852394-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7852394-Restriction Mapping,
pubmed-meshheading:7852394-Templates, Genetic,
pubmed-meshheading:7852394-Transcription, Genetic,
pubmed-meshheading:7852394-Transcription Factors,
pubmed-meshheading:7852394-Transcriptional Activation
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pubmed:year |
1995
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pubmed:articleTitle |
Differential transcriptional activation in vitro by NF-kappa B/Rel proteins.
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pubmed:affiliation |
Terry Fox Molecular Oncology Group, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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