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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-3-14
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pubmed:abstractText |
RME1 is a zinc-finger protein homolog that functions as a repressor of the meiotic activator IME1. RME1 is unusual among yeast repressors in two respects: it acts over a considerable distance (2 kbp) and it can activate transcription from a binding site separated from its natural flanking region. To identify genes required for RME1 to exert repression, we have selected mutants with improved RME1-dependent activation. One rare mutant was defective in RME1-dependent repression of an artificial reporter gene as well as the native IME1 gene. The mutation permits sporulation of a/a diploids, which express RME1 from its natural promoter, and of a/alpha diploids constructed to express RME1 from the GAL1 promoter. The mutation also causes temperature-sensitive growth and a methionine or cysteine requirement. Analysis of a complementing genomic clone indicates that the mutation lies in a known essential gene, RGR1. Prior studies have indicated a functional relationship between RGR1 and SIN4 (also called TSF3); we have found that a sin4 null mutation also causes a defect in RME1-dependent repression and a methionine or cysteine requirement. The rgr1 and sin4 mutations do not cause a reduction of RME1 polypeptide levels. The defect in RME1-dependent repression may result from effects of sin4 and, presumably, rgr1 on chromatin structure.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-1100845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-1406639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-1936989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2076816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2183020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2183032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2196447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2209544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2247050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2659436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2664470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2674657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2828175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2830478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2834270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-2849508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-3280136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-3280971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-3305158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-3540601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-6092916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-6301690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-770230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-8005433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-8177171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-8339935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-8367297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-8389567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-8417990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-8423805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7851756-8455605
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
138
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pubmed:geneSymbol |
HIS3,
IME1,
RES1-1,
RGR1,
RME1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
577-86
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:7851756-Cloning, Molecular,
pubmed-meshheading:7851756-Fungal Proteins,
pubmed-meshheading:7851756-Gene Expression Regulation, Fungal,
pubmed-meshheading:7851756-Genes, Fungal,
pubmed-meshheading:7851756-Genes, Regulator,
pubmed-meshheading:7851756-Genetic Complementation Test,
pubmed-meshheading:7851756-Genetic Linkage,
pubmed-meshheading:7851756-Mediator Complex,
pubmed-meshheading:7851756-Mutation,
pubmed-meshheading:7851756-Plasmids,
pubmed-meshheading:7851756-Repressor Proteins,
pubmed-meshheading:7851756-Saccharomyces cerevisiae,
pubmed-meshheading:7851756-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:7851756-Trans-Activators,
pubmed-meshheading:7851756-Zinc Fingers
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pubmed:year |
1994
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pubmed:articleTitle |
Requirement for RGR1 and SIN4 in RME1-dependent repression in Saccharomyces cerevisiae.
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pubmed:affiliation |
Department of Microbiology, Columbia University, New York, New York 10032.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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