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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-3-13
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pubmed:abstractText |
Prodigiosin 25-C inhibited the accumulation of 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine and acridine orange in the acidic compartments of baby hamster kidney cells with little perturbation of cellular ATP levels. In rat liver lysosomes, prodigiosin 25-C inhibited the proton pump activity with an IC50 of approximately 30 nM, but did not affect ATPase activity up to 1 microM. It also delayed the transport of vesicular stomatitis virus G protein and induced a drastic swelling of Golgi apparatus and mitochondria. These results indicate that prodigiosin 25-C raises the pH of acidic compartments through inhibition of the proton pump activity of vacuolar type H(+)-ATPase, thereby causing the functional and morphological changes to the Golgi apparatus.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
359
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
53-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7851530-Adenosine Triphosphate,
pubmed-meshheading:7851530-Animals,
pubmed-meshheading:7851530-Cell Line,
pubmed-meshheading:7851530-Cricetinae,
pubmed-meshheading:7851530-Glycoproteins,
pubmed-meshheading:7851530-Golgi Apparatus,
pubmed-meshheading:7851530-Hydrogen-Ion Concentration,
pubmed-meshheading:7851530-Immunosuppressive Agents,
pubmed-meshheading:7851530-Kidney,
pubmed-meshheading:7851530-Liver,
pubmed-meshheading:7851530-Lysosomes,
pubmed-meshheading:7851530-Male,
pubmed-meshheading:7851530-Microscopy, Electron,
pubmed-meshheading:7851530-Mitochondrial Swelling,
pubmed-meshheading:7851530-Prodigiosin,
pubmed-meshheading:7851530-Proton Pumps,
pubmed-meshheading:7851530-Proton-Translocating ATPases,
pubmed-meshheading:7851530-Rats,
pubmed-meshheading:7851530-Rats, Wistar,
pubmed-meshheading:7851530-Vacuoles
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pubmed:year |
1995
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pubmed:articleTitle |
Prodigiosin 25-C uncouples vacuolar type H(+)-ATPase, inhibits vacuolar acidification and affects glycoprotein processing.
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pubmed:affiliation |
Department of Bioengineering, Tokyo Institute of Technology, Kanagawa, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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