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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-3-10
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pubmed:abstractText |
In the present study, the inhibitory effect of the cGMP analog (Rp)-8-(para-chlorophenylthio)guanosine-3',5'-cyclic monophosphorothioate ((Rp)-8-pCPT-cGMPS) on the cGMP-dependent protein kinase-mediated protein phosphorylation in intact human platelets was investigated. In vitro phosphorylation experiments with the substrate kemptide demonstrated an inhibition of the cGMP-dependent protein kinase by (Rp)-8-pCPT-cGMPS with a Ki of 0.5 microM. In intact human platelets, (Rp)-8-pCPT-cGMPS antagonized the activation of the cGMP-dependent protein kinase by 8-pCPT-cGMP without affecting cAMP-dependent protein kinase or cGMP-regulated phosphodiesterases. The data obtained suggest that (Rp)-8-pCPT-cGMPS may be a useful tool for studying the role of cGMP in vitro and in intact cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-2999
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
265-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7851503-Animals,
pubmed-meshheading:7851503-Blood Platelets,
pubmed-meshheading:7851503-Blotting, Western,
pubmed-meshheading:7851503-Cattle,
pubmed-meshheading:7851503-Chemistry, Physical,
pubmed-meshheading:7851503-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:7851503-Cyclic GMP,
pubmed-meshheading:7851503-Cyclic GMP-Dependent Protein Kinases,
pubmed-meshheading:7851503-Enzyme Activation,
pubmed-meshheading:7851503-Humans,
pubmed-meshheading:7851503-Oligopeptides,
pubmed-meshheading:7851503-Phosphorylation,
pubmed-meshheading:7851503-Physicochemical Phenomena,
pubmed-meshheading:7851503-Platelet Aggregation Inhibitors,
pubmed-meshheading:7851503-Thionucleotides
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pubmed:year |
1994
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pubmed:articleTitle |
(Rp)-8-pCPT-cGMPS, a novel cGMP-dependent protein kinase inhibitor.
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pubmed:affiliation |
Universität Würzburg, Labor für Klin. Biochemie, Germany.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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