Linked Life Data

7850796

Source:http://linkedlifedata.com/resource/pubmed/id/7850796

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-3-14
pubmed:abstractText
Retinoids, a group of natural and synthetic vitamin A analogues the receptors of which belong to the superfamily of steroid receptors, can exert profound effects on growth and/or differentiation of embryonic and neoplastic cells. Kaposi's sarcoma (KS), previously a rare multicentric neoplasm, has become epidemic with HIV infection, although the etiology of KS remains obscure. In the present study, the effects of two potent retinoids, all-trans-retinoic acid (RA) and 13-cis-RA, on the expression of retinoic acid receptor alpha and the growth of AIDS-related KS (AIDS-KS) cells were examined. The proliferation of AIDS-KS cells was significantly inhibited by RA and 13-cis-RA in a dose-dependent manner with 50% inhibitory concentration of 1.4 x 10(-10) M and 4.7 x 10(-9) M, respectively, which correlate with their potency. Growth inhibition was time dependent with maximal inhibition of 90% after 3 days of treatment with 10(-8) M RA. Growth inhibition by RA was further potentiated by forskolin (1 microM), an intracellular cyclic AMP-inducing agent. Moreover, RA treatment blocked the proliferative effect of oncostatin M and tumor necrosis factor alpha, two major KS autocrine growth factors. The effects of RA were accompanied by a dramatic increase in nuclear staining for retinoic acid receptor alpha and in the relative number of strongly positive retinoic acid receptor alpha nuclei. Finally, RA induced morphological changes as KS cells became more flattened, better spread, and more adhesive to the substrate. These results suggest that retinoids inhibit proliferation of AIDS-KS cells and further support their utility as therapeutic agents in AIDS-KS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
823-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7850796-Acquired Immunodeficiency Syndrome, pubmed-meshheading:7850796-Animals, pubmed-meshheading:7850796-Cell Adhesion, pubmed-meshheading:7850796-Cell Division, pubmed-meshheading:7850796-Cell Nucleus, pubmed-meshheading:7850796-Cyclic AMP, pubmed-meshheading:7850796-Drug Synergism, pubmed-meshheading:7850796-Growth Inhibitors, pubmed-meshheading:7850796-Growth Substances, pubmed-meshheading:7850796-Humans, pubmed-meshheading:7850796-Kinetics, pubmed-meshheading:7850796-Mice, pubmed-meshheading:7850796-Oncostatin M, pubmed-meshheading:7850796-Peptides, pubmed-meshheading:7850796-Rats, pubmed-meshheading:7850796-Receptors, Retinoic Acid, pubmed-meshheading:7850796-Sarcoma, Kaposi, pubmed-meshheading:7850796-Tretinoin, pubmed-meshheading:7850796-Tumor Cells, Cultured, pubmed-meshheading:7850796-Tumor Necrosis Factor-alpha
pubmed:year
1995
pubmed:articleTitle
Inhibition of AIDS-Kaposi's sarcoma cell proliferation following retinoic acid receptor activation.
pubmed:affiliation
Department of Pathology, University of Montreal, Quebec, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't