Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1995-3-8
pubmed:abstractText
Transcriptional activator proteins interact with the general transcription factors TATA-binding protein (TBP), TFIIB and/or other TBP-associated factors (TAFs). Using affinity chromatography we demonstrate that members of the Jun family of transcriptional activators interact with both TBP and TFIIB in vitro. TBP binds to both the N-terminal activation domain and C-terminal bZIP regions of c-Jun, whereas TFIIB binds to only the c-Jun bZIP domain. This interaction requires the dimerization of the Jun protein. The ability of the N-terminal activation domains of c-Jun, JunB, JunD and v-Jun to interact with TBP in vitro correlates with their transcriptional activity in vivo. Domain mapping experiments indicate that c-Jun interacts with the conserved C-terminus of TBP. Studies using a set of TFIIB inframe deletion mutants demonstrate that C-terminal amino acids 178-201 and 238-316 play an important role in modulating the interaction between TFIIB and c-Jun. Although phosphorylation of the c-Jun N-terminal activation domain stimulates c-Jun transcriptional activity in vivo, it has no effect on the ability of c-Jun to interact with either TBP or TFIIB in vitro. These data suggest that the Jun family of activator proteins may activate transcription by interacting with the general transcription factors TBP and TFIIB.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1318414, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1324919, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1328860, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1436073, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1495986, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1496019, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1516835, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1546314, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1608942, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1630458, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1658706, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1661258, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1730659, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1751545, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1828892, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1833071, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1876184, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1903181, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1906140, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1922364, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-1922387, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2001592, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2121368, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2193231, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2194289, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2194667, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2363050, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2374612, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2494702, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2510934, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2513128, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2513129, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-2518690, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-3047011, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-3289117, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-7678780, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-7685215, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-7690963, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8223437, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8224842, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8330735, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8383624, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8384354, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8413585, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8434021, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8464492, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8464496, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8497252, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8504927, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8514746, http://linkedlifedata.com/resource/pubmed/commentcorrection/7848298-8515819
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
305 ( Pt 3)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
967-74
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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