Linked Life Data

7846549

Source:http://linkedlifedata.com/resource/pubmed/id/7846549

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-3-3
pubmed:abstractText
By using [3H] cortisol and [3H] dexamethasone (DEX), a class of specific binding sites of glucocorticoids (GC) in rat liver cell membrane was observed. These binding sites had saturability, high affinity and low capacity. The kinetic parameters of [3H] cortisol and [3H] DEX were: Kd = 12.84 +/- 6.58 nmol/L (n = 4) and 40.27 +/- 23.44 nmol/L (n = 3) respectively; Bmax = 2.57 +/- 1.84 pmol/mg protein and 0.64 +/- 0.18 pmol/mg protein respectively (mean +/- SE). The Kd obtained from the kinetic studies was corresponding to the results of Scatchard analysis. Scatchard plots from the equilibrium experiments showed the straight line. The Hill coefficients were 0.9880 and 0.9990 respectively. The competitive displacement experiments indicated that cortisol possessed the higher specificity for the [3H] cortisol binding sites than other tested steroids (prednisolone, progesterone, RU486, DEX). The competitive ability of cortisol was at least 40 folds higher than mentioned steroids. By means of autoradiography technique, the existence of [3H] cortisol labelled particles on the liver cell membrane was found.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0371-0874
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
488-94
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
[Specific binding sites of [3H] cortisol and [3H] dexamethasone in rat liver cell membrane].
pubmed:affiliation
Endocrinology Research Laboratory, Third Hospital, Beijing Medical University.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't