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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-3-3
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pubmed:abstractText |
The effects of a GLUT4 mini-transgene (containing 7 kb of 5' flanking and 1 kb of 3' flanking sequence and all exons and introns of the GLUT4 gene as well as a small foreign DNA tag) and of exercise training on expression of GLUT4 and glycemic control in mice were investigated. Transgenic mice harboring the minigene expressed < or = 2-fold the normal level of GLUT4 mRNA and protein in skeletal (gastrocnemius) muscle and adipose tissue. This modest tissue-specific increase in GLUT4 expression led to an unexpectedly rapid blood glucose clearance rate following oral glucose administration. In nontransgenic animals exercise caused a 1.5-fold increase in expression of GLUT4 mRNA and protein as well as a significant improvement of glycemic control. In transgenic animals harboring the minigene exercise increased expression of GLUT4 mRNA and protein derived from the minigene and endogenous gene and led to a further improvement of glycemic control. These findings indicate that the cis-regulatory element(s) controlling exercise-induced expression of the GLUT4 gene is located within the nucleotide sequence encompassed by the GLUT4 minigene. The fact that glycemic control is markedly improved by a relatively low level of expression of GLUT4 caused by the transfected minigene and is further enhanced by exercise in transgenic animals demonstrates that GLUT4 plays a pivotal role in glucose homeostasis in vivo. Of the effectors--i.e., cAMP, insulin, and arachidonic acid--known to down-regulate expression of GLUT4 by 3T3-L1 adipocytes in culture, only the decline in circulating arachidonate level in vivo correlated with up-regulation of GLUT4 caused by exercise.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-1534819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-1559408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-1601840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-1628766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-1636703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-1705711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-1991574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-1999488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-2175551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-222468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-2245878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-2354749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-6771756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-6989818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-7030826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-7506491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-7678005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-8248251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-8276864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-8421683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7846068-8475079
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 4,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a4 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
92
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pubmed:geneSymbol |
GLUT4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
865-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:7846068-Animals,
pubmed-meshheading:7846068-Arachidonic Acid,
pubmed-meshheading:7846068-Cyclic AMP,
pubmed-meshheading:7846068-Female,
pubmed-meshheading:7846068-Gene Dosage,
pubmed-meshheading:7846068-Gene Expression Regulation,
pubmed-meshheading:7846068-Glucose,
pubmed-meshheading:7846068-Glucose Tolerance Test,
pubmed-meshheading:7846068-Glucose Transporter Type 4,
pubmed-meshheading:7846068-Homeostasis,
pubmed-meshheading:7846068-Insulin,
pubmed-meshheading:7846068-Male,
pubmed-meshheading:7846068-Mice,
pubmed-meshheading:7846068-Mice, Transgenic,
pubmed-meshheading:7846068-Monosaccharide Transport Proteins,
pubmed-meshheading:7846068-Muscle Proteins,
pubmed-meshheading:7846068-Organ Specificity,
pubmed-meshheading:7846068-Physical Conditioning, Animal,
pubmed-meshheading:7846068-RNA, Messenger
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pubmed:year |
1995
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pubmed:articleTitle |
Expression of an insulin-responsive glucose transporter (GLUT4) minigene in transgenic mice: effect of exercise and role in glucose homeostasis.
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pubmed:affiliation |
Division of Clinical Nutrition, National Institute of Health and Nutrition, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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