rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
12
|
pubmed:dateCreated |
1995-3-7
|
pubmed:abstractText |
The structural characterization of two synthetic model peptides of the cI434 repressor is described. Unequivocal determination of the structure was achieved by means of electrospray ionization mass spectrometry of the intact peptides and by fast atom bombardment mass spectrometric identification of complementary peptide fragments obtained by tryptic and chymotrypic digestion and partial separation by reversed-phase high-performance liquid chromatography. The results show the potential of this approach for characterizing synthetic peptides of relatively high molecular weight.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
1052-9306
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
23
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
727-33
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:7841207-Amino Acid Sequence,
pubmed-meshheading:7841207-Chromatography, High Pressure Liquid,
pubmed-meshheading:7841207-Chymotrypsin,
pubmed-meshheading:7841207-DNA-Binding Proteins,
pubmed-meshheading:7841207-Mass Spectrometry,
pubmed-meshheading:7841207-Molecular Sequence Data,
pubmed-meshheading:7841207-Peptides,
pubmed-meshheading:7841207-Protein Conformation,
pubmed-meshheading:7841207-Repressor Proteins,
pubmed-meshheading:7841207-Spectrometry, Mass, Fast Atom Bombardment,
pubmed-meshheading:7841207-Trypsin,
pubmed-meshheading:7841207-Viral Proteins
|
pubmed:year |
1994
|
pubmed:articleTitle |
Structural characterization of synthetic model peptides of the DNA-binding cI434 repressor by electrospray ionization and fast atom bombardment mass spectrometry.
|
pubmed:affiliation |
International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|