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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1995-3-2
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pubmed:abstractText |
The mutations in the quinolone resistance-determining region of the gyrA and gyrB genes from 27 clinical isolates of Escherichia coli with a range of MICs of ciprofloxacin from 0.007 to 128 micrograms/ml and of nalidixic acid from 2 to > 2,000 micrograms/ml were determined by DNA sequencing. All 15 isolates with ciprofloxacin MICs of > or = 1 micrograms/ml showed a change in Ser-83 to Leu of GyrA protein, whereas in clinical isolates with a MIC of > or = 8 micrograms/ml (11 strains), a double change in Ser-83 and Asp-87 was found. All isolates with a MIC of nalidixic acid of > or = 128 micrograms/ml showed a mutation at amino acid codon Ser-83. Only 1 of the 27 clinical isolates of E. coli analyzed showed a change in Lys-447 of the B subunit of DNA gyrase. A change in Ser-83 is sufficient to generate a high level of resistance to nalidixic acid, whereas a second mutation at Asp-87 in the A subunit of DNA gyrase may play a complementary role in developing the strain's high levels of ciprofloxacin resistance.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-1337067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-1510409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-1656869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-1657531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-1850970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-1850972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-2039202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-2168148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-2500854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-2536729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-2546585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-2548439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-2658782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-2679373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-2828631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-2830458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-2982149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-3000292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-3020376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-3031051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-3056253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-3132091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-3521490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-3532944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-8137121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-8381633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-8384814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-8388197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7840592-8392306
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0066-4804
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2477-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7840592-Amino Acid Sequence,
pubmed-meshheading:7840592-Base Sequence,
pubmed-meshheading:7840592-Ciprofloxacin,
pubmed-meshheading:7840592-DNA Gyrase,
pubmed-meshheading:7840592-DNA Topoisomerases, Type II,
pubmed-meshheading:7840592-Drug Resistance, Microbial,
pubmed-meshheading:7840592-Escherichia coli,
pubmed-meshheading:7840592-Microbial Sensitivity Tests,
pubmed-meshheading:7840592-Molecular Sequence Data,
pubmed-meshheading:7840592-Mutation
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pubmed:year |
1994
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pubmed:articleTitle |
Association between double mutation in gyrA gene of ciprofloxacin-resistant clinical isolates of Escherichia coli and MICs.
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pubmed:affiliation |
Hospital Clínic, Departament de Microbiologia, Facultat de Medicina, Universitat de Barcelona, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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