Linked Life Data

7840176

Source:http://linkedlifedata.com/resource/pubmed/id/7840176

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1 Pt 1
pubmed:dateCreated
1995-2-28
pubmed:abstractText
Different methods for the estimation of whole body protein synthesis (PS) in healthy adult humans were simultaneously compared in seven young adult subjects (6 males, 1 female) fed for 6 days a diet providing 1 g protein.kg-1.day-1 and approximately 188 kJ.kg-1.day-1. A 24-h intravenous tracer study with L-[1-13C]leucine was performed starting at 6 P.M. on day 6. During fasted (6 h) and fed (6 h) steady states, PS was estimated using an approach based on 13CO2 excretion (PSexcr) and on urinary nitrogen excretion data (corrected for changes in body urea pool). Simultaneously, we used the conventional two-pool model and plasma [13C]ketoisocaproate enrichment for estimating PS. The latter mean estimates of PS were significantly higher than PSexcr during fasting [861 +/- 58 (SD) vs. 663 +/- 160 mg protein.kg-1.6 h-1; P < 0.01] and feeding (985 +/- 63 vs. 779 +/- 127 mg protein.kg-1.6 h-1; P < 0.01) and were much less variable. In hourly small-meal feeding, urinary nitrogen excretion was not a reliable index of body protein oxidation when measured over short periods of 6 h, thereby introducing a lack of precision in PSexcr. We suggest that application of the 13CO2 technique to measure PS in humans is limited by the need for relatively prolonged experimental periods of urine collection and tracer infusion within a given physiological state.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
268
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
E174-84
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Whole body protein synthesis in healthy adult humans: 13CO2 technique vs. plasma precursor approach.
pubmed:affiliation
Laboratory of Human Nutrition and Clinical Research Center, Massachusetts Institute of Technology, Cambridge 02139.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.