Linked Life Data

7840146

Source:http://linkedlifedata.com/resource/pubmed/id/7840146

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1 Pt 1
pubmed:dateCreated
1995-2-28
pubmed:abstractText
Insulin secretion from the islets of Langerhans may be initiated or potentiated by increased phospholipase A2 (PLA2) activity. This patch-clamp study of the insulin-secreting HIT tumor cell line assessed whether inhibition of the ATP-sensitive potassium (KATP) channel, which modulates the secretion-associated beta-cell electrical activity, contributes to the secretory response to PLA2. Exogenous PLA2 (100-1,000 mU/ml) reversibly suppressed KATP channel activity in excised inside-out patches. Similarly, mellitin (0.5-5 micrograms/ml), a bee venom component that increases phospholipid susceptibility to metabolism by PLA2, suppressed KATP channel activity, suggesting that PLA2 is present in excised patches. Adding low concentrations of particular lysophospholipids or arachidonic acid also reduced KATP channel activity in excised inside-out patches. In cell-attached patches, the lysophospholipids had a similar effect, whereas arachidonic acid caused channel stimulation; this latter effect was reversed by cyclooxygenase inhibitors. A recently identified ATP-stimulated PLA2 in beta-cells has been proposed as an important mediator of stimulus-secretion coupling in response to nutrients. The present data illustrating that initial products of PLA2 action on membrane phospholipids reduce KATP channel activity indicate a mechanism that may contribute an early stimulatory signal in this pathway. The observation that metabolism of arachidonate via the cyclooxygenase pathway causes KATP channel stimulation demonstrates a potential counterregulatory mechanism.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
268
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C181-90
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
ATP-sensitive K channel modulation by products of PLA2 action in the insulin-secreting HIT cell line.
pubmed:affiliation
Department of Physiology, University of California Los Angeles School of Medicine 90024.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't