Linked Life Data

7839726

Source:http://linkedlifedata.com/resource/pubmed/id/7839726

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1995-2-27
pubmed:abstractText
We studied a 45-69 lipopeptide obtained by N-terminal modification with a N epsilon-palmitoyl lysine residue of the 45-69 peptide derived from the nef protein of HIV. T cells from animals immunized intraperitoneally with 45-69 lipopeptide proliferated in vitro in the presence of 45-69 peptide while no response was obtained after intraperitoneal immunization with 45-69 peptide. The efficiency of the 45-69 lipopeptide is supported by the covalent association to the N epsilon-palmitoyl lysine moiety. The immunogenicity of the 45-69 lipopeptide or of the unmodified peptide is dependent on the route of immunization but is not related to a mitogenic effect on cells or to an increase of the peptide antigenicity. Moreover, only 45-69 lipopeptide induces the secretion of cytokines such as IL-1, IL-6 and TNF-alpha by peritoneal macrophages. Finally, the use of 45-69 lipopeptide permits the activation of highly purified T cells without the addition of antigen-presenting cells. These results have implications for the formulation of synthetic vaccines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0264-410X
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1209-14
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7839726-Amino Acid Sequence, pubmed-meshheading:7839726-Animals, pubmed-meshheading:7839726-Antigen Presentation, pubmed-meshheading:7839726-Cytokines, pubmed-meshheading:7839726-Gene Products, nef, pubmed-meshheading:7839726-HIV-1, pubmed-meshheading:7839726-Immunization, pubmed-meshheading:7839726-Injections, Intraperitoneal, pubmed-meshheading:7839726-Injections, Subcutaneous, pubmed-meshheading:7839726-Lipoproteins, pubmed-meshheading:7839726-Macrophage Activation, pubmed-meshheading:7839726-Male, pubmed-meshheading:7839726-Molecular Sequence Data, pubmed-meshheading:7839726-Molecular Structure, pubmed-meshheading:7839726-Rats, pubmed-meshheading:7839726-Rats, Inbred Lew, pubmed-meshheading:7839726-T-Lymphocytes, pubmed-meshheading:7839726-Vaccines, Synthetic, pubmed-meshheading:7839726-nef Gene Products, Human Immunodeficiency Virus
pubmed:year
1994
pubmed:articleTitle
Effect of a lipopeptidic formulation on macrophage activation and peptide presentation to T cells.
pubmed:affiliation
Centre d'Immunologie des Maladies Transmissibles et Allergiques, Unité mixte INSERM U167-CNRS 624, Lille, France.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't