Linked Life Data

7838132

Source:http://linkedlifedata.com/resource/pubmed/id/7838132

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-2-28
pubmed:abstractText
At 37 degrees, the specific binding of [3H]SCH23390 to purified adrenal medullary plasma membranes accounted for only 20% of total binding. At 4 degrees, the binding did not saturate; therefore, equilibrium binding constants could not be estimated. Similar results were obtained with 125I-SCH23982, a ligand that exhibits 25-fold higher specific activity, compared with [3H]SCH23390. Of 11 dopamine receptor ligands used, only (+)-SCH23390 and (+/-)-SKF83566 inhibited the binding of [3H]SCH23390, but with very low affinities (IC50 values of 446 and 635 nM, respectively). In striatal membranes, binding of [3H]SCH23390 and of 125I-SCH23982 followed saturation isotherms. [3H]SCH23390 exhibited a Kd of 383 pM and a Bmax of 479 fmol/mg of protein, and 125I-SCH23982 exhibited a Kd of 664 pM and a Bmax of 453 fmol/mg of protein. The radioligand was displaced by the D1-selective compounds (+)-SCH23390 (IC50 of 3 nM), (+/-)-SKF83556 (IC50 of 5 nM), and (+)-SKF38393 (IC50 of 17 nM); spiperone and quinpirole were ineffective. [3H]Spiperone binding to bovine striatal and adrenal medullary plasma membranes exhibited similar characteristics, compatible with a typical D2 receptor. Northern blot analysis revealed the presence of D1 receptor mRNA in poly(A)+ RNA preparations from bovine brain striatum. When Northern blots containing poly(A)+ from bovine adrenal medulla were probed, no specific hybridization band for D1 receptors was observed; in contrast, a band of the expected size for D2 receptors was obtained. Similar results were obtained with in situ hybridization techniques and with more sensitive reverse transcription-polymerase chain reaction methods. The data support the idea that the peripheral D2 receptor present in bovine adrenal medulla is similar to striatal D2 receptors; in contrast, striatal D1 receptors do not seem to have a counterpart in bovine adrenal medullary tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0026-895X
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
40-50
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7838132-2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine, pubmed-meshheading:7838132-Adrenal Medulla, pubmed-meshheading:7838132-Animals, pubmed-meshheading:7838132-Base Sequence, pubmed-meshheading:7838132-Benzazepines, pubmed-meshheading:7838132-Catecholamines, pubmed-meshheading:7838132-Cattle, pubmed-meshheading:7838132-Caudate Nucleus, pubmed-meshheading:7838132-Cell Membrane, pubmed-meshheading:7838132-Corpus Striatum, pubmed-meshheading:7838132-Gene Expression, pubmed-meshheading:7838132-Iodine Radioisotopes, pubmed-meshheading:7838132-Kinetics, pubmed-meshheading:7838132-Microsomes, pubmed-meshheading:7838132-Molecular Sequence Data, pubmed-meshheading:7838132-Radioligand Assay, pubmed-meshheading:7838132-Receptors, Dopamine D1, pubmed-meshheading:7838132-Receptors, Dopamine D2, pubmed-meshheading:7838132-Sequence Homology, Nucleic Acid, pubmed-meshheading:7838132-Spiperone, pubmed-meshheading:7838132-Tritium
pubmed:year
1995
pubmed:articleTitle
Expression of the bovine striatal D2 receptor, but not the D1 receptor, in bovine adrenal medulla.
pubmed:affiliation
Departamento de Fisiología y Farmacología, Facultad de Medicina, Universidad de Salamanca, Spain.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't