Linked Life Data

7836865

Source:http://linkedlifedata.com/resource/pubmed/id/7836865

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-2-27
pubmed:abstractText
Intravitreal daunomycin (D) effectively suppresses cellular proliferation in experimental proliferative vitreoretinopathy (PVR) but has a narrow therapeutic safety range. Studies were undertaken to reduce toxicity of D by preparing a slow-release implant using polysulfone capillary fiber (PCF). Fabrication of the implant involved loading PCF with 1% D in tristearin (w/w), an excipient with diffusion-retardant properties. Two dose levels of the PCF-D device (15 micrograms and 30 micrograms/device) were prepared and sterilized prior to use. To examine the kinetics and efficacy of the device, rabbits were randomized and eyes were implanted as follows: 1) control group (PCF vehicle); 2) PCF-D (15 micrograms/device); 3) PCF-D (30 micrograms/device). Immediately after implantation, all eyes received an intravitreal injection of 2.5 x 10(5) retinal pigmented epithelial (RPE) cells. Thereafter, tractional retinal detachments (TRD) were graded by ophthalmoscopic examination. Also, fluorophotometry scanning from the retina to the anterior chamber was performed to determine the intraocular bioavailability of D. Results showed a therapeutically sustained level of D up to 21 days after device implantation. Midvitreous concentration of D was greater in group 3 than group 2 at all time points examined, indicating a dose-proportional increase in D release. Results of the PVR study showed that by 7 days after treatment, all eyes implanted with the PCF vehicle developed stage 2 TRD or greater; only 1 eye in each of groups 2 and 3 developed stage 2. By 2 weeks, most eyes in groups 2 and 3 remained in stages 1 and 2 with only 2 eyes progressing to stages 3 and 4 TRD. By 5 weeks, all eyes in group 1 showed stages 4 and 5 TRD, while most eyes in groups 2 and 3 remained in stages 1 and 2. The device with 30 micrograms D was more effective in preventing TRD. In conclusion, these data indicate that PCF can reduce the toxicity of D and may be a useful implant for treatment of PVR.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
8756-3320
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
561-70
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Effects of an intravitreal daunomycin implant on experimental proliferative vitreoretinopathy: simultaneous pharmacokinetic and pharmacodynamic evaluations.
pubmed:affiliation
Pharmacia, Inc., Columbus, Ohio.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.