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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
|
pubmed:dateCreated |
1995-2-27
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pubmed:abstractText |
The YTA-1 anti-LFA-1 alpha mAb activates protein tyrosine kinase (PTK), augments NK cytotoxicity, and induces proliferation of fresh CD3- large granular lymphocytes. We demonstrate here that LFA-1 is physically associated in the YT human NK-like cell line cells with a PTK(s) that is distinct from Src family PTKs such as Lck, Fyn, or Lyn. In vitro kinase assays revealed similar association of protein kinase activity with LFA-1 in normal CD3- large granular lymphocytes. Tyrosine phosphorylation of the proteins associated with LFA-1 drastically increased in YT cells after stimulation with NK-sensitive K562 cells but not with NK-resistant P815 cells. Furthermore, pretreatment of YT cells with TS1/22 anti-LFA-1 alpha and TS1/18 anti-LFA-1 beta mAbs abrogated not only the cytotoxicity against K562 cells but also an increase in tyrosine phosphorylation of LFA-1-associated molecules induced by K562 stimulation. These results provide biochemical evidence that the PTK(s) associated with LFA-1 is involved in the signal transduction that follows the recognition of NK target cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
154
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
1691-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7836753-Antibodies, Monoclonal,
pubmed-meshheading:7836753-Cell Line,
pubmed-meshheading:7836753-Cytotoxicity, Immunologic,
pubmed-meshheading:7836753-Enzyme Activation,
pubmed-meshheading:7836753-Humans,
pubmed-meshheading:7836753-Killer Cells, Natural,
pubmed-meshheading:7836753-Lymphocyte Activation,
pubmed-meshheading:7836753-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:7836753-Protein-Tyrosine Kinases,
pubmed-meshheading:7836753-Signal Transduction,
pubmed-meshheading:7836753-Tumor Cells, Cultured
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pubmed:year |
1995
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pubmed:articleTitle |
Stimulation of NK-like YT cells via leukocyte function-associated antigen (LFA)-1. Possible involvement of LFA-1-associated tyrosine kinase in signal transduction after recognition of NK target cells.
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pubmed:affiliation |
Department of Late Effect Studies, Kyoto University, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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