Linked Life Data

7836466

Source:http://linkedlifedata.com/resource/pubmed/id/7836466

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-3-2
pubmed:databankReference
pubmed:abstractText
In primary rodent cells transformed by the E1A region of the highly oncogenic adenovirus type 12, repression of transcription mediated by the far upstream TATA-like element was observed only in conjunction with either possible juxtaposition of a CAA repeated element in the presence of E1A and was dependent upon the relative arrangement of both the TATA-like and CAA repeated motifs in both homologous and heterologous promoter constructs. A gel shift competition study demonstrated that the TATA-binding protein (TBP) or a TBP-like protein can bind to both the upstream TATA-like sequence and the regular TATA box on the H-2Kb basal promoter. Moreover, employing immunoselection and cyclic amplification and selection of targets (CASTing) methods with nuclear extracts derived from Ad12-E1A transformants, we have identified a high affinity binding site in the H-2Kb class I promoter for E1A-associated DNA-binding proteins. The sequences of the binding sites were identified and were found to contain both the upstream TATA-like motif and the CAA repeated motifs. Our results suggest that the TATA-like sequence in the far upstream region of the H-2Kb gene is one of the elements that is required for Ad12-E1A-mediated negative repression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
270
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
2327-36
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7836466-Adenovirus E1A Proteins, pubmed-meshheading:7836466-Animals, pubmed-meshheading:7836466-Base Sequence, pubmed-meshheading:7836466-Binding, Competitive, pubmed-meshheading:7836466-Cell Transformation, Viral, pubmed-meshheading:7836466-DNA-Binding Proteins, pubmed-meshheading:7836466-Gene Expression Regulation, pubmed-meshheading:7836466-H-2 Antigens, pubmed-meshheading:7836466-Mice, pubmed-meshheading:7836466-Molecular Sequence Data, pubmed-meshheading:7836466-Promoter Regions, Genetic, pubmed-meshheading:7836466-RNA, Messenger, pubmed-meshheading:7836466-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:7836466-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:7836466-Repressor Proteins, pubmed-meshheading:7836466-TATA Box, pubmed-meshheading:7836466-Transcription, Genetic
pubmed:year
1995
pubmed:articleTitle
Cooperatively between an upstream TATA-like sequence and a CAA repeated element mediates E1A-dependent negative repression of the H-2Kb class I gene.
pubmed:affiliation
Tsukuba Life Science Center, RIKEN (Institute of Physical and Chemical Research), Ibaraki, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't