rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0040132,
umls-concept:C0040160,
umls-concept:C0205171,
umls-concept:C0300926,
umls-concept:C0337051,
umls-concept:C0380603,
umls-concept:C0392337,
umls-concept:C0596235,
umls-concept:C1509144,
umls-concept:C2917322
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pubmed:issue |
2
|
pubmed:dateCreated |
1995-3-2
|
pubmed:abstractText |
TSH-induced intracellular Ca2+ concentration ([Ca2+]i) changes in single FRTL-5 thyroid cells were analyzed by digital video imaging of fura-2-loaded cells. More than 80% of the cells responded to as little as 30 nM TSH, resulting in a [Ca2+]i rise in the presence and absence of extracellular Ca2+. One micromolar concentration of N6-(L-2-phenylisopropyl)adenosine (PIA) caused no appreciable [Ca2+]i increase in more than 300 cells examined, but induced the [Ca2+]i elevation in more than 90% of the cells that had previously been treated with TSH. Pertussis toxin treatment abolished the PIA, but not the TSH, action, whereas thapsigargin-induced Ca2+ depletion of the inositol 1,4,5-trisphosphate-sensitive pool inhibited the actions of both TSH and PIA. The time courses of Ca2+ response considerably differentiated among single cells, but showed similarity among different areas in each cell. These results suggest that PIA induces Ca2+ release from the same thapsigargin-sensitive pool as that targeted by TSH. The lack of PIA stimulation of basic fibroblast growth factor-induced phospholipase-C gamma activation as well as the rise in [Ca2+]i suggests that the cooperative PIA action occurs specifically on phospholipase-C beta.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Purinergic P1 Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P1,
http://linkedlifedata.com/resource/pubmed/chemical/Terpenes,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/Thyrotropin,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0013-7227
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
136
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
770-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:7835309-Animals,
pubmed-meshheading:7835309-Calcium,
pubmed-meshheading:7835309-Cells, Cultured,
pubmed-meshheading:7835309-Fibroblast Growth Factors,
pubmed-meshheading:7835309-Fura-2,
pubmed-meshheading:7835309-Image Processing, Computer-Assisted,
pubmed-meshheading:7835309-Pertussis Toxin,
pubmed-meshheading:7835309-Purinergic P1 Receptor Agonists,
pubmed-meshheading:7835309-Rats,
pubmed-meshheading:7835309-Receptors, Purinergic P1,
pubmed-meshheading:7835309-Sensitivity and Specificity,
pubmed-meshheading:7835309-Terpenes,
pubmed-meshheading:7835309-Thapsigargin,
pubmed-meshheading:7835309-Thyroid Gland,
pubmed-meshheading:7835309-Thyrotropin,
pubmed-meshheading:7835309-Time Factors,
pubmed-meshheading:7835309-Virulence Factors, Bordetella
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pubmed:year |
1995
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pubmed:articleTitle |
Cooperation of thyrotropin, but not basic fibroblast growth factor, with an adenosine receptor agonist in Ca2+ mobilization from thapsigargin-sensitive pools in single FRTL-5 thyroid cells.
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pubmed:affiliation |
Laboratory of Signal Transduction, Gunma University, Maebashi, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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