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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-2-28
|
| pubmed:abstractText |
Renal cell carcinoma (RCC) is one human tumor to which the immune response may control the growth of tumor cells. These tumors are infiltrated by a large mononuclear infiltrate mainly composed of T lymphocytes. To characterize the lymphocytes infiltrating RCC, we analyzed the molecular structure of the T cell receptor (TCR) alpha and beta chains in tumor and paired peripheral blood lymphocytes from a series of 6 untreated patients. We first determined V alpha and V beta gene segment usage by PCR using a panel of V specific oligonucleotide primers (V alpha 1-w29 and V beta 1-w24). A highly diverse usage of TCR V alpha and V beta gene usage was observed in 5 of 6 tumors. In addition, the few tumor overexpressed V beta specificities detected by reverse transcription-PCR were shown to contain minor T cell expansions. Strikingly, 1 of the 6 tumor studied displayed a skewed TCR repertoire with V beta 4 transcript representing 25% of the TCR signals. Clonality of the tumor overexpressed V beta transcripts was analyzed by CDR3 size distribution analysis. In the particular tumor displaying a biased repertoire large expansions of T cell subpopulations were detected (particularly in V beta 4) specifically at the tumor site. Such T cells may be expanded locally in response to tumor antigens.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0008-5472
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
55
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
685-90
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7834641-Adult,
pubmed-meshheading:7834641-Aged,
pubmed-meshheading:7834641-Amino Acid Sequence,
pubmed-meshheading:7834641-Antigens, CD3,
pubmed-meshheading:7834641-Base Sequence,
pubmed-meshheading:7834641-Carcinoma, Renal Cell,
pubmed-meshheading:7834641-Cloning, Molecular,
pubmed-meshheading:7834641-DNA Primers,
pubmed-meshheading:7834641-Female,
pubmed-meshheading:7834641-Humans,
pubmed-meshheading:7834641-Kidney Neoplasms,
pubmed-meshheading:7834641-Lymph Node Excision,
pubmed-meshheading:7834641-Lymph Nodes,
pubmed-meshheading:7834641-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:7834641-Male,
pubmed-meshheading:7834641-Middle Aged,
pubmed-meshheading:7834641-Molecular Sequence Data,
pubmed-meshheading:7834641-Nephrectomy,
pubmed-meshheading:7834641-Polymerase Chain Reaction,
pubmed-meshheading:7834641-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:7834641-T-Lymphocyte Subsets,
pubmed-meshheading:7834641-Transcription, Genetic
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
In vivo local expansion of clonal T cell subpopulations in renal cell carcinoma.
|
| pubmed:affiliation |
Laboratoire d'Immunologie Cellulaire, INSERM U333, Institut Gustave Roussy, Villejuif, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|