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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1995-3-1
|
| pubmed:abstractText |
We report a simple and rapid method for DNA preparation suitable for PCR by microwave irradiation. When mouse whole blood and hair shafts were directly irradiated and subjected to PCR, a unique gene such as the retinoblastoma susceptibility gene was reproducibly amplified. This method was useful for screening a transgene sequence in transgenic mice.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0736-6205
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
17
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
726, 728
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7833036-Animals,
pubmed-meshheading:7833036-Base Sequence,
pubmed-meshheading:7833036-Hair,
pubmed-meshheading:7833036-Mice,
pubmed-meshheading:7833036-Mice, Transgenic,
pubmed-meshheading:7833036-Microwaves,
pubmed-meshheading:7833036-Molecular Sequence Data,
pubmed-meshheading:7833036-Polymerase Chain Reaction
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Direct PCR of whole blood and hair shafts by microwave treatment.
|
| pubmed:affiliation |
Medical Research Institute, Nihon University School of Medicine, Tokyo, Japan.
|
| pubmed:publicationType |
Technical Report
|