Linked Life Data

7832717

Source:http://linkedlifedata.com/resource/pubmed/id/7832717

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-2-17
pubmed:abstractText
Immunoglobulins were separated from ostrich sera by ammonium sulfate precipitation and ion-exchange chromatography. Two classes of immunoglobulin could be identified, corresponding to IgG and IgM of other species, based on elution profiles from ion-exchange columns and molecular mass estimation on gel-filtration chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). On SDS-PAGE, the heavy chains of IgG and IgM were shown to have molecular masses of 67.5 kDa and 65 kDa, respectively, and the light chains common to both were shown to have a molecular mass of 27 kDa. The ostrich immunoglobulins were not recognized by monoclonal and polyclonal antibodies against immunoglobulins of many species of animals tested, nor by antibodies against chicken immunoglobulins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0005-2086
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
616-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Isolation and characterization of serum immunoglobulin classes of the ostrich (Struthio camelus).
pubmed:affiliation
Department of Biochemistry, University of Zimbabwe, Mount Pleasant, Harare.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't