Linked Life Data

7832534

Source:http://linkedlifedata.com/resource/pubmed/id/7832534

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1995-2-17
pubmed:abstractText
Polyphosphate metabolism in Escherichia coli was studied in order to determine the role of polyphosphates in energy and phosphate metabolism. Phosphate-shift experiments were performed on wild-type E. coli W3110 and on an E. coli strain mutant in the genes encoding the polyphosphate-metabolizing enzymes polyphosphate kinase (PPK) and polyphosphatase (PPX). The levels of polyphosphates were measured by [31P]NMR, and the activities of PPK and PPX were measured using enzymatic assays. During phosphate starvation, the intracellular level of polyphosphate was not detectable in E. coli W3110; the activities of PPX and alkaline phosphatase were high relative to those during exponential growth. During the shift from phosphate starvation to phosphate surplus conditions, PPX activity decreased and PPK activity and intracellular polyphosphate stores increased dramatically. These results imply an important role for polyphosphates in cellular energy and phosphate storage and in adaptation to adverse growth conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0077-8923
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
745
pubmed:geneSymbol
phoT, phoU, ppk, pstA, pstB
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
77-91
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Polyphosphate metabolism in Escherichia coli.
pubmed:affiliation
Department of Chemical Engineering, University of California, Berkeley 94720-9989.
pubmed:publicationType
Journal Article