|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0010453,
umls-concept:C0015127,
umls-concept:C0030685,
umls-concept:C0033268,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0041491,
umls-concept:C0086022,
umls-concept:C0206679,
umls-concept:C0391871,
umls-concept:C0442335,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1314792,
umls-concept:C1705099,
umls-concept:C1963578
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1995-2-21
|
|
pubmed:abstractText |
In this report we demonstrate that a defective herpes simplex virus type one (HSV-1) vector can express enzymatically active tyrosine hydroxylase in cultured striatal cells that are thereby converted into L-DOPA-producing cells. A human tyrosine hydroxylase cDNA (form II) was inserted into an HSV-1 vector (pHSVth) and packaged into virus particles using an HSV-1 strain 17 mutant in the immediate early 3 gene (either ts K or D30EBA) as helper virus. Cultured fibroblasts were infected with pHSVth and 1 day later tyrosine hydroxylase immunoreactivity and tyrosine hydroxylase enzyme activity were observed. The tyrosine hydroxylase enzyme activity directed the production of L-DOPA. pHSVth infection of striatal cells in dissociated cell culture resulted in expression of tyrosine hydroxylase RNA and tyrosine hydroxylase immunoreactivity. Release of L-DOPA and low levels of dopamine were observed from cells in pHSVth-infected striatal cultures. Expression of tyrosine hydroxylase and release of catecholamines were maintained for at least 1 week after infection.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-1311846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-1345766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-1494905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-1650784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-1662813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-169103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-1704416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-1721735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-1970430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-1976199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-1976413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2105529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2119630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2153970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2167931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2173924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2174168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2443800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2571152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2573072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2761778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2838826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-284369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2843986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2886883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2892528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2896667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-2897424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-3279301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-3279303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-3760948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-4400965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-5820999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-6166661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-6252286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-6327885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-8096625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-8100096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-8101641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-8102799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-8215983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-8382740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-8386379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7830040-8392189
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0022-3042
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
64
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
487-96
|
|
pubmed:dateRevised |
2010-12-17
|
|
pubmed:meshHeading |
pubmed-meshheading:7830040-Humans,
pubmed-meshheading:7830040-Animals,
pubmed-meshheading:7830040-Rats,
pubmed-meshheading:7830040-Corpus Striatum,
pubmed-meshheading:7830040-Catecholamines,
pubmed-meshheading:7830040-Fibroblasts,
pubmed-meshheading:7830040-Cells, Cultured,
pubmed-meshheading:7830040-Levodopa,
pubmed-meshheading:7830040-Rats, Sprague-Dawley,
pubmed-meshheading:7830040-Defective Viruses
|
