Linked Life Data

7829587

Source:http://linkedlifedata.com/resource/pubmed/id/7829587

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-2-17
pubmed:abstractText
Establishing regulatory mechanisms that mediate proliferation of osteoblasts while restricting expression of genes associated with mature bone cell phenotypic properties to post-proliferative cells is fundamental to understanding skeletal development. To gain insight into relationships between growth control and the developmental expression of genes during osteoblast differentiation, we have examined expression of three classes of genes during the cell cycle of normal diploid rat calvarial-derived osteoblasts and rat osteosarcoma cells (ROS 17/2.8): cell cycle and growth-related genes (e.g., histone), genes that encode major structural proteins (e.g., actin and vimentin), and genes related to the biosynthesis, organization, and mineralization of the bone extracellular matrix (e.g., alkaline phosphatase, collagen I, osteocalcin, and osteopontin). In normal diploid osteoblasts as well as in osteosarcoma cells we found that histone genes, required for cell progression, are selectively expressed during S phase. All other genes studied were constitutively expressed both at the transcriptional and posttranscriptional levels. Alkaline phosphatase, an integral membrane protein in both osteoblasts and osteosarcoma cells, exhibited only minimal changes in activity during the osteoblast and osteosarcoma cell cycles. Our findings clearly indicate that despite the loss of normal proliferation-differentiation interrelationships in osteosarcoma cells, cell cycle regulation or constitutive expression of growth and phenotypic genes is maintained.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0730-2312
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
274-82
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7829587-Actins, pubmed-meshheading:7829587-Alkaline Phosphatase, pubmed-meshheading:7829587-Animals, pubmed-meshheading:7829587-Bone Neoplasms, pubmed-meshheading:7829587-Bone and Bones, pubmed-meshheading:7829587-Cell Cycle, pubmed-meshheading:7829587-Cell Division, pubmed-meshheading:7829587-Cell Line, pubmed-meshheading:7829587-Cells, Cultured, pubmed-meshheading:7829587-Collagen, pubmed-meshheading:7829587-Diploidy, pubmed-meshheading:7829587-Extracellular Matrix, pubmed-meshheading:7829587-Fetus, pubmed-meshheading:7829587-Gene Expression, pubmed-meshheading:7829587-Histones, pubmed-meshheading:7829587-Osteoblasts, pubmed-meshheading:7829587-Osteocalcin, pubmed-meshheading:7829587-Osteopontin, pubmed-meshheading:7829587-Osteosarcoma, pubmed-meshheading:7829587-Phenotype, pubmed-meshheading:7829587-Rats, pubmed-meshheading:7829587-Sialoglycoproteins, pubmed-meshheading:7829587-Skull, pubmed-meshheading:7829587-Tumor Cells, Cultured, pubmed-meshheading:7829587-Vimentin
pubmed:year
1994
pubmed:articleTitle
Expression of cell growth and bone phenotypic genes during the cell cycle of normal diploid osteoblasts and osteosarcoma cells.
pubmed:affiliation
Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01655.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't