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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1995-2-22
|
| pubmed:databankReference | |
| pubmed:abstractText |
Complete cDNAs encoding human mitochondrial translational initiation factor 2 (IF-2mt) have been obtained from liver, heart, and fetal brain cDNA libraries. These cDNAs have a long open reading frame 2181 residues in length encoding a protein of 727 amino acids. Overall, human IF-2mt has 30-40% identity to the corresponding prokaryotic factors. Surprisingly, it is no more homologous to yeast IF-2mt than to the IF-2s from bacterial sources. The greatest region of conservation lies in the G-domain of this factor with less conservation in the COOH-terminal half of the protein and very little homology near the amino terminus. The 5'-untranslated leaders of the liver and heart cDNAs contain a number of short open reading frames. These sequences may play a role in the translational activity of the IF-2mt mRNA. Northern analysis indicates that the IF-2mt gene is expressed in all tissues but that the level of expression varies over a wide range.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
27
|
| pubmed:volume |
270
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1859-65
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7829522-Amino Acid Sequence,
pubmed-meshheading:7829522-Animals,
pubmed-meshheading:7829522-Bacteria,
pubmed-meshheading:7829522-Base Sequence,
pubmed-meshheading:7829522-Brain,
pubmed-meshheading:7829522-Cloning, Molecular,
pubmed-meshheading:7829522-Conserved Sequence,
pubmed-meshheading:7829522-DNA, Complementary,
pubmed-meshheading:7829522-DNA Primers,
pubmed-meshheading:7829522-Fetus,
pubmed-meshheading:7829522-Gene Library,
pubmed-meshheading:7829522-Hominidae,
pubmed-meshheading:7829522-Humans,
pubmed-meshheading:7829522-Liver,
pubmed-meshheading:7829522-Mitochondria,
pubmed-meshheading:7829522-Molecular Sequence Data,
pubmed-meshheading:7829522-Myocardium,
pubmed-meshheading:7829522-Open Reading Frames,
pubmed-meshheading:7829522-Peptide Initiation Factors,
pubmed-meshheading:7829522-Polymerase Chain Reaction,
pubmed-meshheading:7829522-Prokaryotic Initiation Factor-2,
pubmed-meshheading:7829522-Saccharomyces cerevisiae,
pubmed-meshheading:7829522-Sequence Homology, Amino Acid
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Cloning and sequence analysis of the human mitochondrial translational initiation factor 2 cDNA.
|
| pubmed:affiliation |
Department of Chemistry, University of North Carolina, Chapel Hill 27599.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|