Linked Life Data

7828063

Source:http://linkedlifedata.com/resource/pubmed/id/7828063

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-2-23
pubmed:abstractText
With the aim of limiting peptide purification steps and unambiguously ascertaining protein sequences, we have designed and implemented on a personal computer an algorithm to determine sequence alignment by utilizing data obtained from automatic Edman degradation performed on a single peptide mixture and individual peptides. The protein under study is digested by two different hydrolysis methods and fragments are just isolated from one mixture and sequenced, while the second mixture is submitted unfractionated to sequence analysis. The algorithm provides for the exact alignment of the individual peptides using the mixture data for the overlapping. We report an example of application of this approach by utilizing experimental data obtained from a protein of known sequence.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0266-7061
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
489-94
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
An algorithm to determine protein sequence alignment by utilizing data obtained from a peptide mixture and individual peptides.
pubmed:affiliation
Dipartimento di Agrobiologia ed Agrochimica, Università della Tuscia, Viterbo, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't