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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
1995-2-22
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pubmed:abstractText |
Inadequate co-stimulation of tumor reactive T cells may contribute to the fact that antigenic tumors are not normally rejected by the immune system. We recently reported that the induction of profound unresponsiveness in a T cell clone by melanoma cells expressing MHC class II antigens may provide a mechanism for these tumor cells to escape immunosurveillance. Here we demonstrate that two T cell clones (sTC3 and sTS5) produced high amounts of IL-10 after being rendered anergic by autologous melanoma cells. Co-culture of these T cell clones with melanoma cell transfectants expressing B7, which failed to induce anergy, resulted in a significantly lower production of IL-10. IL-10 production by the anergic T cell clones correlated with an impaired ability to produce IL-2 in response to TCR mediated activation. Neutralization of IL-10 reduced the duration of T cell unresponsiveness from 14 to only 4 days, but inhibition of IL-10 production during initiation of anergy showed no effect on it. Induction of the unresponsive state, as well as the subsequent IL-10 production in sTC3 cells, could be prevented by the addition of cyclosporin A to the primary co-culture of sTC3 and the autologous melanoma. Taken together, these results indicate that IL-10 is important for maintenance of T cell anergy induced by contact with nonprofessional antigen presenting cells such as MHC class II+ melanoma cells. Furthermore, we were able to detect IL-10 in serum from melanoma patients and IL-10 mRNA in tumor infiltrating lymphocytes isolated from melanoma metastases, suggesting an in vivo relevance of our in vitro findings.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0953-8178
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1605-12
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:7826950-Antigens, CD3,
pubmed-meshheading:7826950-Base Sequence,
pubmed-meshheading:7826950-Clonal Anergy,
pubmed-meshheading:7826950-Clone Cells,
pubmed-meshheading:7826950-HLA-D Antigens,
pubmed-meshheading:7826950-Humans,
pubmed-meshheading:7826950-Interleukin-10,
pubmed-meshheading:7826950-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:7826950-Melanoma,
pubmed-meshheading:7826950-Molecular Sequence Data,
pubmed-meshheading:7826950-Polymerase Chain Reaction,
pubmed-meshheading:7826950-T-Lymphocytes,
pubmed-meshheading:7826950-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Maintenance of clonal anergy by endogenously produced IL-10.
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pubmed:affiliation |
Institute of Dermatology, University of Würzburg, Germany.
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pubmed:publicationType |
Journal Article
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