Linked Life Data

7826364

Source:http://linkedlifedata.com/resource/pubmed/id/7826364

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-2-16
pubmed:abstractText
The purpose of this study was to identify the active oxygen species generated in murine skin fibroblasts under UVB irradiation. When fibroblasts were exposed to UV light (UVA + UVB), hydroxyl radicals were detectable by ESR-spin trapping using DMPO as a spin trapping agent. Cell damage induced by UVB irradiation was prevented by addition of DMSO, a typical hydroxyl radical scavenger. Fibroblasts treated with a catalase inhibitor 3-amino-1H-1,2,4-triazole were more sensitive to UVB irradiation than untreated-cells, but the sensitivity was improved by addition of DMSO. To find the origin of the hydroxyl radicals in fibroblasts during UVB irradiation, H2O2 was determined by dihydrorhodamine 123 and it was found that the formation of H2O2 increased under UVB irradiation. These results suggest that UVB-irradiated skin fibroblasts produce H2O2 and were followed by the formation of hydroxyl radicals, probably derived from the Fenton-like reaction (H2O2 + Fe2+ --> .OH + OH- + Fe3+), which in turn damages the cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
206
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
474-9
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation.
pubmed:affiliation
Shiga Central Laboratory, Noevir Co., LTD., Japan.
pubmed:publicationType
Journal Article