Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-2-16
|
| pubmed:databankReference | |
| pubmed:abstractText |
Triggering of Ag receptors on lymphocytes induces rapid phosphorylation of several receptor-associated protein tyrosine kinases (PTKs), implicating their role in controlling cellular growth and differentiation. In this study, we report the cloning of a human cDNA encoding a nonreceptor PTK with a calculated M(r) of about 58 kDa. The kinase has an overall amino acid identity of approximately 87% with the murine Blk. However, in the unique domain there is only 58% homology and an insertion of six amino acids in the N-terminal region. The nature of this insertion suggests a functional role in membrane attachment. Northern blot analysis showed expression in all stages of B cell development and in T cell lines. The message was not observed in the nonlymphoid tissues examined. In contrast, expression of murine blk in plasma cells and T lymphocytes has not been reported. Importantly, transcripts were seen in human embryonic liver as early as 7.5 wk of gestation before the rearrangement of Ig H chain locus. Furthermore, transcripts were detected in human thymocytes and not in mature T cells. Southern blot analysis revealed polymorphism of this gene in a Caucasian population but not in a Gambian population, indicating a recent origin of this polymorphism. The gene was localized to chromosome 8p22-23. The homology at the protein level suggests that this kinase may be the human homologue of murine Blk. Expression of BLK in immature T cells suggests that BLK may play an important role in thymopoiesis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
154
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1265-72
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:7822795-Amino Acid Sequence,
pubmed-meshheading:7822795-Base Sequence,
pubmed-meshheading:7822795-Blotting, Southern,
pubmed-meshheading:7822795-Cell Differentiation,
pubmed-meshheading:7822795-Chromosome Mapping,
pubmed-meshheading:7822795-Cloning, Molecular,
pubmed-meshheading:7822795-Genomic Library,
pubmed-meshheading:7822795-Humans,
pubmed-meshheading:7822795-In Situ Hybridization, Fluorescence,
pubmed-meshheading:7822795-Molecular Sequence Data,
pubmed-meshheading:7822795-Polymerase Chain Reaction,
pubmed-meshheading:7822795-Protein Biosynthesis,
pubmed-meshheading:7822795-Protein-Tyrosine Kinases,
pubmed-meshheading:7822795-Sequence Homology, Amino Acid,
pubmed-meshheading:7822795-T-Lymphocytes,
pubmed-meshheading:7822795-Tumor Cells, Cultured,
pubmed-meshheading:7822795-src-Family Kinases
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Molecular cloning, characterization, and chromosomal localization of a human lymphoid tyrosine kinase related to murine Blk.
|
| pubmed:affiliation |
Center for BioTechnology, Karolinska Institute, NOVUM, Huddinge, Sweden.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|