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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0009017,
umls-concept:C0017262,
umls-concept:C0086418,
umls-concept:C0205314,
umls-concept:C0262950,
umls-concept:C0284930,
umls-concept:C0679622,
umls-concept:C1171362,
umls-concept:C1515670,
umls-concept:C2717970
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pubmed:issue |
1
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pubmed:dateCreated |
1995-2-9
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pubmed:databankReference |
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pubmed:abstractText |
We have previously cloned a rabbit cDNA clone (OC-2) from an osteoclast cDNA library by the differential screening. OC-2 was found to encode a novel cysteine proteinase, tentatively called cathepsin K, which is predominantly expressed in osteoclasts. By use of a rabbit OC-2 fragment as a probe, its human counterpart was cloned from a cDNA library of osteoarthritic hip bone. The cloned human cDNA (hOC-2) encoded a protein of 329 amino acid residues and its deduced amino acid sequence showed 94% homology to rabbit cathepsin K. Multiple alignment of amino acid sequences of human cathepsins B, H, L, S and K showed the highest homology of cathepsin K to cathepsin S 48%. Northern blot analysis showed that cathepsin K mRNA is expressed at high levels in some osteoarthritic hip bones and at a very high level in osteoclastoma compared to very low levels in other tissues. These results suggest that cathepsin K is closely involved in human osteoclastic bone resorption.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-291X
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
206
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
89-96
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7818555-Amino Acid Sequence,
pubmed-meshheading:7818555-Animals,
pubmed-meshheading:7818555-Base Sequence,
pubmed-meshheading:7818555-Bone and Bones,
pubmed-meshheading:7818555-Cathepsin K,
pubmed-meshheading:7818555-Cathepsins,
pubmed-meshheading:7818555-Cloning, Molecular,
pubmed-meshheading:7818555-Consensus Sequence,
pubmed-meshheading:7818555-DNA, Complementary,
pubmed-meshheading:7818555-Gene Expression,
pubmed-meshheading:7818555-Gene Library,
pubmed-meshheading:7818555-Humans,
pubmed-meshheading:7818555-Molecular Sequence Data,
pubmed-meshheading:7818555-Organ Specificity,
pubmed-meshheading:7818555-Osteoarthritis,
pubmed-meshheading:7818555-Osteoblasts,
pubmed-meshheading:7818555-RNA, Messenger,
pubmed-meshheading:7818555-Rabbits,
pubmed-meshheading:7818555-Sequence Homology, Amino Acid
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pubmed:year |
1995
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pubmed:articleTitle |
Molecular cloning of human cDNA for cathepsin K: novel cysteine proteinase predominantly expressed in bone.
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pubmed:affiliation |
International Research Laboratories, Ciba-Geigy Japan Ltd., Takarazuka.
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pubmed:publicationType |
Journal Article,
Comparative Study
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