|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0013879,
umls-concept:C0017337,
umls-concept:C0020792,
umls-concept:C0021665,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0086860,
umls-concept:C0205147,
umls-concept:C0205314,
umls-concept:C0679622,
umls-concept:C1336789
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1995-2-9
|
|
pubmed:abstractText |
Although the major endocrine source of insulin-like growth factor-I (IGF-I) is the liver, little is known about IGF-I transcriptional mechanisms in this tissue. To evaluate the role of cis-regulatory elements, rat hepatocytes in primary culture were transfected with DNA constructs containing IGF-I 5'flanking sequences, fused to a luciferase reporter. We demonstrate the presence of a novel promoter approximately 0.5 kb upstream from exon 1 transcription initiation sites, together with a repressor element in this region, and a downstream repressor element which can modulate the activity of both endogenous and heterologous promoters.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0006-291X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
5
|
|
pubmed:volume |
206
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
279-86
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:7818532-Animals,
pubmed-meshheading:7818532-Base Sequence,
pubmed-meshheading:7818532-Cells, Cultured,
pubmed-meshheading:7818532-Consensus Sequence,
pubmed-meshheading:7818532-Exons,
pubmed-meshheading:7818532-Humans,
pubmed-meshheading:7818532-Insulin-Like Growth Factor I,
pubmed-meshheading:7818532-Liver,
pubmed-meshheading:7818532-Luciferases,
pubmed-meshheading:7818532-Male,
pubmed-meshheading:7818532-Molecular Sequence Data,
pubmed-meshheading:7818532-Promoter Regions, Genetic,
pubmed-meshheading:7818532-Rats,
pubmed-meshheading:7818532-Rats, Sprague-Dawley,
pubmed-meshheading:7818532-Recombinant Fusion Proteins,
pubmed-meshheading:7818532-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7818532-Repressor Proteins,
pubmed-meshheading:7818532-Sequence Homology, Nucleic Acid,
pubmed-meshheading:7818532-Transcription, Genetic,
pubmed-meshheading:7818532-Transfection
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
Identification of novel promoter and repressor elements in the 5'-flanking regions of the rat insulin-like growth factor-I gene.
|
|
pubmed:affiliation |
Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|
