Linked Life Data

7817047

Source:http://linkedlifedata.com/resource/pubmed/id/7817047

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-2-9
pubmed:abstractText
The mammalian diaphragm (Dia) is a unique skeletal muscle because of its chronic contractile activity. Chronically active muscles have higher capacities for glycogen synthesis from lactate and glucose. In this study, the contribution of alanine to glycogen synthesis in the predominantly fast-twitch mouse Dia was determined. Chronically active fast- and slow-twitch muscles from dy2J/dy2J pseudomyotonic mice were also analyzed. Alanine aminotransferase (AlaAT) activity was significantly (P < 0.05) greater in Dia and chronically active gastrocnemius muscle (Gast) than control Gast. 14C-label incorporation into glycogen following in vivo injection of 14C-alanine, was significantly higher in Dia and chronically active Gast than control Gast. A direct incorporation of 14C from 14C-alanine into glycogen is also observed in vitro. The incorporation rate shows a linear concentration dependent relationship and a pH optimum of 6.3. Insulin had no effect on glycogen synthesis from alanine by Dia in vitro. The extensor digitorum longus muscle (EDL) and chronically active EDL and soleus muscle (Sol) had higher rates of glycogen synthesis from alanine than control Sol and Dia. The oxidation of alanine to CO2 was the primary route for alanine metabolism by the Dia. These results demonstrate that the Dia can synthesize glycogen from alanine, but only at low rates.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0034-5687
pubmed:author
pubmed:issnType
Print
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-64
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Limited capacity for glyconeogenesis from alanine by diaphragm muscle.
pubmed:affiliation
Department of Zoology, Louisiana State University, Baton Rouge 70803.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, U.S. Gov't, Non-P.H.S.