Linked Life Data

7815919

Source:http://linkedlifedata.com/resource/pubmed/id/7815919

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-2-8
pubmed:abstractText
This study examined the role of antibodies against the X-protein, a surface-localized antigen frequently associated with streptococci causing mastitis in cattle, in the opsonization and phagocytosis of unencapsulated Streptococcus agalactiae. The analysis of various strains of serotype NT/X by flow cytometry, after labeling with a monoclonal antibody to X-protein, revealed that they consisted of a mixture of unstained and stained bacteria. Cloning of mother strains yielded clones of unstained bacteria but not homogeneous clones of stained bacteria. Analysis by ELISA of an unstained clone (4.1) derived from the reference NT/X strain 24/60 indicated that it expressed low amount of X-protein at its surface, about 25 times less than the stained clone 24/60 5.6. Colloidal gold immunolabeling showed the X-protein at the periphery of bacteria (of clone 5.6 and in lower amount of clone 4.1), at a distance from the cell wall. Bovine antibodies (essentially IgG) to X-protein behaved like the monoclonal antibody in the cytometric assay. They activated the classical pathway of complement as shown by the deposition of C1q and C4 on bacteria. Deposition of C4 also occurred on the low-surface-producing clone 4.1 in the presence of antibodies to X-protein, although less efficiently than on the high-surface-producing clone 5.6. When used alone, antibodies promoted the ingestion of bacteria and heat-inactivated immune serum promoted the chemiluminescence activity and the killing by polymorphonuclear cells. In conclusion, antibodies to X-protein induced the deposition of C3 by the classical pathway and were also able to stimulate opsonophagocytic killing of X-bearing S. agalactiae in the absence of deposited C3.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0882-4010
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
359-72
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7815919-Animals, pubmed-meshheading:7815919-Antibodies, Bacterial, pubmed-meshheading:7815919-Antigenic Variation, pubmed-meshheading:7815919-Antigens, Bacterial, pubmed-meshheading:7815919-Bacterial Outer Membrane Proteins, pubmed-meshheading:7815919-Cattle, pubmed-meshheading:7815919-Complement System Proteins, pubmed-meshheading:7815919-Female, pubmed-meshheading:7815919-Flow Cytometry, pubmed-meshheading:7815919-Gene Expression Regulation, Bacterial, pubmed-meshheading:7815919-Immunohistochemistry, pubmed-meshheading:7815919-Mastitis, Bovine, pubmed-meshheading:7815919-N-Acetylneuraminic Acid, pubmed-meshheading:7815919-Neutrophils, pubmed-meshheading:7815919-Opsonin Proteins, pubmed-meshheading:7815919-Phagocytosis, pubmed-meshheading:7815919-Phenotype, pubmed-meshheading:7815919-Serotyping, pubmed-meshheading:7815919-Sialic Acids, pubmed-meshheading:7815919-Streptococcal Infections, pubmed-meshheading:7815919-Streptococcus agalactiae
pubmed:year
1994
pubmed:articleTitle
Phenotypic variability of X-protein expression by mastitis-causing Streptococcus agalactiae of serotype NT/X and opsonic activities of specific antibodies.
pubmed:affiliation
Laboratoire de Pathologie Infectieuse et d'Immunologie, Centre de Recherches de Tours, Nouzilly, France.
pubmed:publicationType
Journal Article, Comparative Study