Linked Life Data

7815740

Source:http://linkedlifedata.com/resource/pubmed/id/7815740

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1995-2-8
pubmed:abstractText
We conducted a study to investigate whether expression of the contractile proteins in cultured rat mesangial cells (MC) was associated with the cell cycle and protein kinase C (PKC). When growth-arrested MC were stimulated with 100 nM phorbol myristate acetate (PMA) for 24 hours, an increased expression of smooth muscle alpha-actin and vimentin was detected by immunocytochemistry. A proportion of the S- and G2/M-phase in MC was increased in accordance with the enhanced expression of contractile proteins on flow cytometry. Immunoblot analysis revealed that 100 nM PMA stimulated expression of alpha-actin and vimentin as a single band. These results indicate that expression of contractile proteins, such as alpha-actin and vimentin, is dependent on the cell cycle and PKC, suggesting a phenotypic change in which MC assume smooth muscle cell characteristics.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0385-2385
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1090-4
pubmed:dateRevised
2011-8-1
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
[Association of cell cycle and protein kinase C with the expression of cytoskeletal protein in cultured rat mesangial cells].
pubmed:affiliation
Department of Medicine, Kidney Center, Tokyo Women's Medical college, Japan.
pubmed:publicationType
Journal Article, English Abstract