Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-2-9
pubmed:abstractText
Mouse hepatitis virus strain A59 encodes a papain-like cysteine proteinase (PLP-1) that, during translation of ORF1a, cleaves p28 from the amino terminus of the growing polypeptide chain. In order to determine the amino acid sequences surrounding the p28 cleavage site, the first 4.6 kb of murine hepatitis virus strain A59 ORF1a was expressed in a cell-free transcription-translation system. Amino-terminal radiosequencing of the resulting downstream cleavage product demonstrated that cleavage occurs between Gly-247 and Val-248. Site-directed mutagenesis of amino acids surrounding the p28 cleavage site revealed that substitutions of Arg-246 (P2) and Gly-247 (P1) nearly eliminated cleavage of p28. Single-amino-acid substitutions of other residues between P7 and P2' were generally permissive for cleavage, although a few changes did greatly reduce proteolysis. The relationship between the p28 cleavage site and other viral and cellular papain proteinase cleavage sites is discussed.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-1318604, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-1331507, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-1652473, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-1736533, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-1846489, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-1902114, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-1918054, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-1966414, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-1996319, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-2141206, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-2142454, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-2159623, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-2545027, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-2547993, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-2656254, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-2824826, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-3018279, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-3045756, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-8032266, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-8209734, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-8291254, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-8337838, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-8396668, http://linkedlifedata.com/resource/pubmed/commentcorrection/7815547-8420970
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
809-13
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Identification of the murine coronavirus p28 cleavage site.
pubmed:affiliation
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 94104-6076.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.