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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-2-9
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pubmed:databankReference |
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pubmed:abstractText |
The mechanism of action of hepatitis B virus (HBV) X protein in transcriptional transactivation and in tumorigenesis remains obscure. We have used the yeast two-hybrid system to identify a cellular protein that can interact with HBV X protein. This protein, designated X-associated protein 1 (XAP-1), is a human homolog of the UV-damaged DNA-binding protein (UV-DDB) recovered from a monkey cell cDNA library. UV-DDB is presumed to be involved in DNA repair. The interaction between X protein and XAP-1 protein was verified by immunoprecipitation of yeast cell lysates expressing both proteins and by in vitro mixing with X protein expressed as a glutathione S-transferase fusion protein and XAP-1 protein either in HeLa cell extracts or synthesized by in vitro translation. We speculate that the interaction of X protein with a DNA repair protein may recruit cellular proteins to repair the partially double-stranded HBV genome or may modify cellular transcription processes. An effect on the cellular DNA repair system may explain a cofactor role for HBV in liver cancer development.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-1317597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-1322242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-1325733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-1549357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-1583465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-1638635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-1731101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-1827531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-2034275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-2125986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-2142553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-2243380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-2547163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-3002015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-3033659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-3938367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-6324998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8107266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8128251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8134379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8145827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8171034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8242751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8249276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8252616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8261515,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8367466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8371985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8384581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8386823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8394683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8431446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8437213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8441471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8465201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-8483493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7815490-89756
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1107-14
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7815490-Amino Acid Sequence,
pubmed-meshheading:7815490-Animals,
pubmed-meshheading:7815490-Base Sequence,
pubmed-meshheading:7815490-Cells, Cultured,
pubmed-meshheading:7815490-DNA Repair,
pubmed-meshheading:7815490-DNA-Binding Proteins,
pubmed-meshheading:7815490-Humans,
pubmed-meshheading:7815490-Liver Neoplasms,
pubmed-meshheading:7815490-Molecular Sequence Data,
pubmed-meshheading:7815490-Rabbits,
pubmed-meshheading:7815490-Trans-Activators,
pubmed-meshheading:7815490-Yeasts
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pubmed:year |
1995
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pubmed:articleTitle |
Hepatitis B virus X protein interacts with a probable cellular DNA repair protein.
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pubmed:affiliation |
Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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