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rdf:type |
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lifeskim:mentions |
umls-concept:C0016263,
umls-concept:C0019682,
umls-concept:C0019699,
umls-concept:C0032520,
umls-concept:C0229664,
umls-concept:C0332307,
umls-concept:C0444498,
umls-concept:C0681850,
umls-concept:C0806987,
umls-concept:C0936012,
umls-concept:C1510438,
umls-concept:C1514468,
umls-concept:C1550501,
umls-concept:C1706203,
umls-concept:C2349001,
umls-concept:C2697811
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pubmed:issue |
9
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pubmed:dateCreated |
1995-2-6
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pubmed:abstractText |
The presence of human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood mononuclear cells (PBMC) of three groups (group 1, more than 500 CD4+ T cells per microliter; group 2, between 200 and 499 CD4+ T cells per microliter; group 3, fewer than 200 CD4+ T cells per microliter) of HIV-1-infected patients, in different stages of the disease, was determined by using a newly developed flow cytometry analysis of the product of in situ PCR assay and compared with other markers of viral replication (HIV-1 p24 antigenemia and viral isolation). Results showed varied percentages of HIV-1-infected PBMC, ranging from 0.6 to 20%. Patients with more than 500 CD4+ T cells per microliter showed the lowest percentage of HIV-1-infected PBMC (2.1 +/- 1.7), compared with patients with CD4+ T-cell counts of between 200 and 499 per microliter (6.5% +/- 4.1%; P < 0.001) and patients with fewer than 200 CD4+ T cells per microliter (4.9% +/- 4.7%; P < 0.05). The difference in the percentage of HIV-1-infected PBMC between group 2 and group 3 patients may in part reflect the loss of CD4+ T lymphocytes in more advanced stages of the disease. However, the results clearly indicate a striking coincidence between the fall of the CD4+ T-cell count below 400/microliter and the sharp increase in PBMC virus loading and p24 antigenemia. Since the procedure is relatively easy to perform, it could be used to monitor the evolution of HIV-1 infection and may prove a useful adjunct in tailoring therapeutic strategies.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-1336322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-1364173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-1569974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-1579465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-1672549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-1678399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-1682922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-1974752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-2296085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-2482842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-2573256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-2665081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-2981635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-2989831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-3014036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-3336784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-7496917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-7904167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-7905630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-8093551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-8096068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-8103340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-8280406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-8280422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-8427715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-8455722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814539-8493534
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2152-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7814539-AIDS-Related Complex,
pubmed-meshheading:7814539-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:7814539-Base Sequence,
pubmed-meshheading:7814539-CD4 Lymphocyte Count,
pubmed-meshheading:7814539-DNA, Viral,
pubmed-meshheading:7814539-Flow Cytometry,
pubmed-meshheading:7814539-HIV Core Protein p24,
pubmed-meshheading:7814539-HIV Seropositivity,
pubmed-meshheading:7814539-HIV-1,
pubmed-meshheading:7814539-Humans,
pubmed-meshheading:7814539-Leukocytes, Mononuclear,
pubmed-meshheading:7814539-Molecular Sequence Data,
pubmed-meshheading:7814539-Polymerase Chain Reaction,
pubmed-meshheading:7814539-Viremia
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pubmed:year |
1994
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pubmed:articleTitle |
Quantification of human immunodeficiency virus type 1-infected mononuclear cells in peripheral blood of seropositive subjects by newly developed flow cytometry analysis of the product of an in situ PCR assay.
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pubmed:affiliation |
Institute of Microbiology, University of Bologna Medical School, St. Orsola General Hospital, Italy.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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