Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1995-2-6
pubmed:abstractText
Two criteria must be met before mycobacterial specimens can be tested by DNA amplification methods: (i) the sample must be rendered noninfectious, and (ii) the organisms must be lysed to free the DNA. Previous publications reporting DNA amplification of mycobacteria have concentrated on lysis and amplification procedures and have not addressed the issue of sample safety. We have shown that heating of samples below 100 degrees C may not consistently kill mycobacteria; however, heating at 100 degrees C in a boiling-water bath or a forced-air oven for a minimum of 5 min kills mycobacteria, including Mycobacterium thermoresistibile. Furthermore, heating at 100 degrees C for 30 min consistently lyses mycobacteria to produce short fragments of DNA that are suitable for amplification by PCR and strand displacement amplification. This procedure works with clinical samples digested by the n-acetyl cysteine-NaOH method as well as with suspensions of organisms in phosphate buffer. This paper also demonstrates the feasibility of using strand displacement amplification with clinical specimens.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-1309614, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-1402031, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-1579461, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-1622262, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-1761699, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-1791860, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-1908051, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-1928175, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-1952448, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-2108994, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-2109022, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-2116445, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-2123884, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-2155396, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-2572798, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-2892795, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-2999980, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-3286624, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-4967201, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-8370752, http://linkedlifedata.com/resource/pubmed/commentcorrection/7814537-8506155
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2140-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:7814537-Acetylcysteine, pubmed-meshheading:7814537-Bacteriolysis, pubmed-meshheading:7814537-Base Sequence, pubmed-meshheading:7814537-Buffers, pubmed-meshheading:7814537-Containment of Biohazards, pubmed-meshheading:7814537-DNA, Bacterial, pubmed-meshheading:7814537-Disinfectants, pubmed-meshheading:7814537-Feasibility Studies, pubmed-meshheading:7814537-Hot Temperature, pubmed-meshheading:7814537-Humans, pubmed-meshheading:7814537-Molecular Sequence Data, pubmed-meshheading:7814537-Mycobacterium, pubmed-meshheading:7814537-Mycobacterium Infections, pubmed-meshheading:7814537-Nucleic Acid Amplification Techniques, pubmed-meshheading:7814537-Polymerase Chain Reaction, pubmed-meshheading:7814537-Safety, pubmed-meshheading:7814537-Sensitivity and Specificity, pubmed-meshheading:7814537-Sodium Hydroxide, pubmed-meshheading:7814537-Time Factors
pubmed:year
1994
pubmed:articleTitle
Rendering of mycobacteria safe for molecular diagnostic studies and development of a lysis method for strand displacement amplification and PCR.
pubmed:affiliation
Veterans Affairs Medical Center, Durham, North Carolina 27705.
pubmed:publicationType
Journal Article, Comparative Study