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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1995-2-9
|
| pubmed:abstractText |
Forty patients with chronic liver disease and HCC were analyzed for infection with hepatitis C (HCV) and hepatitis B (HBV) viruses. All patients were negative for HBsAg, 16 were alcoholics, 6 had previous blood transfusions and 18 had sporadic chronic hepatitis. Antibodies to HCV were determined by EIA 2nd generation. HBV-DNA was detected by PCR using primers of the precore region. Analysis of HCV-RNA was done with nested PCR amplifying the 5' non-coding region of the HCV genome, using primers complementary to nucleotides 1-20 and 305-320 and nested primers complementary to nucleotides 21-31 and 271-286 of the HCV-J1. Anti-HCV were positive in 35/40 patients (87.5%). HCV-RNA was detected by PCR in 34 patients (85%) all of them positive for the anti-HCV. HCV-RNA was detected in 70.5% of the alcohol abusers, in 100% of patients with history of transfusion(s) and 94.1% of patients with cryptogenic chronic liver disease. HBV-DNA was detected in only 2 patients. In conclusion, there is a high rate of HCV and a low rate of HBV viremia detected by PCR in Spanish patients with HCC HBsAg negative. No patient without anti-HCV presents HCV-RNA. Our results suggest that persistent HCV replication may play a role in hepatic carcinogenesis, as HBV-DNA could be found in only 5% of our HCC patients.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0393-2990
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
189-94
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7813697-Aged,
pubmed-meshheading:7813697-Base Sequence,
pubmed-meshheading:7813697-Carcinoma, Hepatocellular,
pubmed-meshheading:7813697-Chronic Disease,
pubmed-meshheading:7813697-DNA, Viral,
pubmed-meshheading:7813697-Female,
pubmed-meshheading:7813697-Hepacivirus,
pubmed-meshheading:7813697-Hepatitis B,
pubmed-meshheading:7813697-Hepatitis B Surface Antigens,
pubmed-meshheading:7813697-Hepatitis B virus,
pubmed-meshheading:7813697-Hepatitis C,
pubmed-meshheading:7813697-Humans,
pubmed-meshheading:7813697-Liver Diseases,
pubmed-meshheading:7813697-Liver Neoplasms,
pubmed-meshheading:7813697-Male,
pubmed-meshheading:7813697-Molecular Sequence Data,
pubmed-meshheading:7813697-Polymerase Chain Reaction,
pubmed-meshheading:7813697-RNA, Viral,
pubmed-meshheading:7813697-Virus Replication
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Demonstration of HCV-RNA and HBV-DNA in the serum of HBsAg negative patients with hepatocellular carcinoma.
|
| pubmed:affiliation |
Department of Gastroenterology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|