Linked Life Data

7813618

Source:http://linkedlifedata.com/resource/pubmed/id/7813618

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-2-9
pubmed:abstractText
The effect of lysophosphatidic acid (LPA) on the proliferation of normal and tumor mouse mammary epithelial cells in primary, serum-free, collagen gel cell culture was evaluated. LPA stimulated the growth of normal mammary epithelial cells from mature virgin mice. The growth of pregnancy-dependent tumors (PDT) was generally stimulated, although the response was attenuated in some of these tumors compared to normal cells. In contrast, the growth of 70% of ovarian-independent tumors (OIT) was inhibited by LPA; the remainder were unaffected. LPA stimulated cAMP accumulation and phosphoinositide (PI) hydrolysis in normal, PDT, and OIT. Thus, the regulation of adenylyl cyclase and PI-specific phospholipase C by LPA is similar in normal and tumor cells. Pertussis toxin (PT) partially inhibited LPA-stimulated growth in normal cells but did not affect LPA-stimulated PI hydrolysis or cAMP accumulation. Thus, PT-sensitive and -insensitive proliferative pathways are activated. PT also inhibited LPA-stimulated growth of PDT but generally had no effect on the growth of OIT. These results show that the mitogenic response to LPA is attenuated in the hormone-dependent phenotype and switches to growth inhibition in hormone-independent tumors. Furthermore, LPA stimulates multiple signal transduction pathways mediated by PT-sensitive and -insensitive G proteins. The PT-sensitive pathways are not tightly coupled to the proliferative response to LPA in tumor cells. These data suggest that alterations in G protein function may occur during tumor progression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:volume
216
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
178-86
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7813618-Adenylate Cyclase Toxin, pubmed-meshheading:7813618-Animals, pubmed-meshheading:7813618-Cell Division, pubmed-meshheading:7813618-Cells, Cultured, pubmed-meshheading:7813618-Collagen, pubmed-meshheading:7813618-Cyclic AMP, pubmed-meshheading:7813618-Epithelial Cells, pubmed-meshheading:7813618-Epithelium, pubmed-meshheading:7813618-Female, pubmed-meshheading:7813618-Lysophospholipids, pubmed-meshheading:7813618-Mammary Glands, Animal, pubmed-meshheading:7813618-Mammary Neoplasms, Experimental, pubmed-meshheading:7813618-Mice, pubmed-meshheading:7813618-Mice, Inbred BALB C, pubmed-meshheading:7813618-Pertussis Toxin, pubmed-meshheading:7813618-Phosphatidic Acids, pubmed-meshheading:7813618-Phosphatidylinositols, pubmed-meshheading:7813618-Pregnancy, pubmed-meshheading:7813618-Protein Kinase C, pubmed-meshheading:7813618-Signal Transduction, pubmed-meshheading:7813618-Tetradecanoylphorbol Acetate, pubmed-meshheading:7813618-Tumor Cells, Cultured, pubmed-meshheading:7813618-Virulence Factors, Bordetella
pubmed:year
1995
pubmed:articleTitle
Analysis of the proliferative response to lysophosphatidic acid in primary cultures of mammary epithelium: differences between normal and tumor cells.
pubmed:affiliation
Cancer Research Laboratory, University of California, Berkeley 94720.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.