Linked Life Data

7812124

Source:http://linkedlifedata.com/resource/pubmed/id/7812124

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-2-3
pubmed:abstractText
An analytical method has been developed with which to measure the microsomal enzyme activities responsible for oxidative theophylline metabolism. Three metabolites: 3-Methylxanthine (3-MX); 1-methylxanthine (1-MX); 1,3-dimethyluric acid (1,3-DMU), with acetaminophen as an internal standard (IS), were separated by solid phase extraction using a Sep-Pak C18 cartridge, followed by high performance liquid chromatography on a reversed-phase column with isocratic elution using 25 mM acetate buffer containing 4% acetonitrile and 2.5 mM tetra-n-butylammonium hydrogen sulphate (pH 5.25) as the mobile phase. The analytes were clearly resolved and no interference with foreign peaks was observed. A linear relationship was obtained for the metabolites over the concentration range of 0.5-5.0 micrograms/mL, and their analytical recovery was almost 100%. This method can be used to assess drug interactions involving alterations in the biotransformation of theophylline.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0269-3879
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
189-92
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:articleTitle
Measurement of theophylline metabolites produced by reaction with hepatic microsome by high performance liquid chromatography following solid phase extraction.
pubmed:affiliation
Department of Hospital Pharmacy, Shiga University of Medical Science, Ohtsu, Japan.
pubmed:publicationType
Journal Article