Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-2-3
pubmed:abstractText
Infection of B lymphocytes and epithelial tissue by Epstein-Barr virus (EBV) is associated with malignancy and autoimmunity. The cellular receptor for EBV has been identified as CD21 (CR2). A molecule, which is biochemically and immunologically similar to B-cell CD21, has been identified on a subpopulation of immature thymocytes, suggesting a role for this molecule in the regulation of T-cell development and further suggesting that immature T cells might be susceptible to EBV infection. A growing body of literature now documents the presence of EBV in tumors of T-cell origin. We have evaluated the susceptibility of the human immature T cell line, HPB-ALL, to infection by EBV. Electron microscopy studies showed a rapid internalization of virus by HPB cells. Southern blotting showed the intracellular presence of linear EBV genomes, and components of the virus replicative cycle were identified. Expression of the BamHI Z region of the genome, encoding the nuclear protein, ZEBRA, which is strictly associated with productive infection in B cells, was detected in HPB-ALL cells. A spliced variant of Z, RAZ, was also identified. Cell surface expression of EBV late antigens was observed to occur transiently. Infection of HPB cells was also accompanied by altered expression of T-cell surface molecules involved in antigen recognition, a process critical to normal development of the T-cell repertoire. Delineation of the outcome of T-cell infection by EBV may lead to a better understanding of the role of this virus in autoimmune processes and malignancy.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
456-64
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7811999-Autoimmune Diseases, pubmed-meshheading:7811999-Base Sequence, pubmed-meshheading:7811999-Cell Line, pubmed-meshheading:7811999-DNA, Viral, pubmed-meshheading:7811999-Gene Expression Regulation, Viral, pubmed-meshheading:7811999-Genome, Viral, pubmed-meshheading:7811999-Herpesvirus 4, Human, pubmed-meshheading:7811999-Humans, pubmed-meshheading:7811999-Microscopy, Electron, pubmed-meshheading:7811999-Molecular Sequence Data, pubmed-meshheading:7811999-Neoplasms, pubmed-meshheading:7811999-Receptors, Complement 3d, pubmed-meshheading:7811999-Receptors, Virus, pubmed-meshheading:7811999-T-Lymphocytes, pubmed-meshheading:7811999-Thymus Gland, pubmed-meshheading:7811999-Tumor Virus Infections, pubmed-meshheading:7811999-Viral Proteins, pubmed-meshheading:7811999-Virus Replication
pubmed:year
1995
pubmed:articleTitle
Model of Epstein-Barr virus infection of human thymocytes: expression of viral genome and impact on cellular receptor expression in the T-lymphoblastic cell line, HPB-ALL.
pubmed:affiliation
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't