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pubmed-article:7811265pubmed:abstractTextHuman proteasomes consist of 14 major subunits which can be resolved by two-dimensional polyacrylamide gel electrophoresis. Tryptic peptides from the subunits were sequenced. This allowed identification of all proteins in two-dimensional electrophoresis gels with proteasome subunits whose sequences are known from cDNA. The results also precisely define the specificity of a panel of subunit-specific monoclonal antibodies. A new proteasome subunit (Z) was discovered. In contrast, the putative subunit MECL-1 could not be detected in human placenta proteasomes.lld:pubmed
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pubmed-article:7811265pubmed:articleTitleHuman proteasome subunits from 2-dimensional gels identified by partial sequencing.lld:pubmed
pubmed-article:7811265pubmed:affiliationAugust Krogh Institute, University of Copenhagen, Denmark.lld:pubmed
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