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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-2-2
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pubmed:abstractText |
Human proteasomes consist of 14 major subunits which can be resolved by two-dimensional polyacrylamide gel electrophoresis. Tryptic peptides from the subunits were sequenced. This allowed identification of all proteins in two-dimensional electrophoresis gels with proteasome subunits whose sequences are known from cDNA. The results also precisely define the specificity of a panel of subunit-specific monoclonal antibodies. A new proteasome subunit (Z) was discovered. In contrast, the putative subunit MECL-1 could not be detected in human placenta proteasomes.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
205
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1785-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7811265-Amino Acid Sequence,
pubmed-meshheading:7811265-Antibodies, Monoclonal,
pubmed-meshheading:7811265-Cysteine Endopeptidases,
pubmed-meshheading:7811265-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:7811265-Female,
pubmed-meshheading:7811265-Humans,
pubmed-meshheading:7811265-Molecular Sequence Data,
pubmed-meshheading:7811265-Multienzyme Complexes,
pubmed-meshheading:7811265-Peptide Fragments,
pubmed-meshheading:7811265-Placenta,
pubmed-meshheading:7811265-Pregnancy,
pubmed-meshheading:7811265-Proteasome Endopeptidase Complex,
pubmed-meshheading:7811265-Protein Conformation,
pubmed-meshheading:7811265-Sequence Homology, Amino Acid
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pubmed:year |
1994
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pubmed:articleTitle |
Human proteasome subunits from 2-dimensional gels identified by partial sequencing.
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pubmed:affiliation |
August Krogh Institute, University of Copenhagen, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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