Linked Life Data

7810609

Source:http://linkedlifedata.com/resource/pubmed/id/7810609

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6 Pt 1
pubmed:dateCreated
1995-1-31
pubmed:abstractText
Ratiometric fura 2 fluorescence techniques were used to investigate the sources of Ca2+ that lead to an increase in cytosolic Ca2+ concentration ([Ca2+]i) and the generation of force during cholinergic stimulation of canine colonic circular smooth muscle tissues. Acetylcholine (ACh; 1 microM) caused a biphasic increase in [Ca2+]i and force. The initial upstroke phase was characterized by an increase in [Ca2+]i and a pronounced increase in force. The sustained phase was characterized by concurrent oscillations in [Ca2+]i and force (2-3/min) that persisted as long as ACh was present. The increase in [Ca2+]i in response to ACh was reduced to approximately 30% in the presence of nicardipine (1 microM), suggesting that L-type Ca2+ channels contribute to the rise in [Ca2+]i but that other sources also contribute. Preincubation in caffeine (10 mM) and ryanodine (10 microM) reduced the upstroke phase of the increase in [Ca2+]i and contractile responses to ACh, indicating that release of Ca2+ from intracellular stores contributes only to the initial cholinergic response. Responses to ACh persisted when nicardipine (1 microM) was included after emptying of caffeine-ryanodine-sensitive stores, suggesting the presence of additional sources of Ca2+. Data suggest that cholinergic regulation of [Ca2+]i in colonic smooth muscle occurs by a number of parallel pathways. Influx through voltage-dependent Ca2+ channels, release of Ca2+ from intracellular stores, and possibly Ca2+ entry through additional conductances activated by ACh all contribute to the regulation of [Ca2+]i.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C1666-73
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Sources of calcium utilized in cholinergic responses in canine colonic smooth muscle.
pubmed:affiliation
Department of Physiology, University of Nevada School of Medicine, Reno 89557.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.