Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
26
pubmed:dateCreated
1995-2-2
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34906, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34907, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34908, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34957, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34958, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34959, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34960, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34961, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34962, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34963, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34964, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34965, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z34966, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z35083, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z35084
pubmed:abstractText
Rheumatoid factor (RF) autoantibodies can be produced in healthy individuals after infections or immunizations and thus escape normal tolerization mechanisms. It has not been clear whether such autoantibodies can undergo somatic hypermutation and affinity maturation similar to antibodies to exogenous antigens. We have investigated how these autoantibodies are regulated in normal individuals by analyzing the sequences of monoclonal IgM RFs obtained as hybridomas from donors after immunization. The variable regions undergo extensive hypermutation, but in contrast to antibodies against exogenous antigens, there is a strong selection against mutations that result in replacement of amino acids in the hypervariable, or complementarity-determining, regions. Furthermore, we found no increase in affinity of these RFs with the accumulation of mutations. This suggests that high-affinity variants are tolerized during the hypermutation process and there is a peripheral mechanism operating on certain autoreactive B cells that, while not deleting or anergizing all autoreactive cells, prevents the generation of high-affinity autoantibodies. Comparison of RFs by using the VH1 DP-10 heavy chain variable region segment from both normal individuals and rheumatoid arthritis (RA) patients suggests that RF from RA patients may not be subject to such a controlling mechanism.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-1348366, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-13681773, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-1404388, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-1427922, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-1469099, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-1578151, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-1591002, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-1693338, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-1703209, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-1900690, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-1900950, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2022732, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2040792, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2107394, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2170450, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2269469, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2416469, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2431452, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2492198, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2578542, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2594086, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2615648, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-2682636, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-3023493, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-3023521, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-3089914, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-3261841, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-3498121, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-3981007, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-4192271, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-4563335, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-6300689, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-6417259, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-6601551, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-668802, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-7509350, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-7686945, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-8056036, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-8099917, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-8157964, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-8228225, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-8262038, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-8268138, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-8397779, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-8428591, http://linkedlifedata.com/resource/pubmed/commentcorrection/7809146-8500520
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12917-21
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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