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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1995-1-27
|
| pubmed:abstractText |
A novel approach to the creation of artificial and modified proteins has been elaborated. The approach includes a sequence design based on the molecular theory of protein secondary structure and folding patterns, gene expression in a cell-free system and testing of structural properties of the synthesized polypeptides at a nanogram level using radiolabelled chains. The approach has been applied to a new synthetic protein albebetin which has been designed to form a 3-D fold which does not contradict any structural rule but has been never observed up to now in natural proteins. Using size-exclusion chromatography, urea-gradient electrophoresis and limited proteolysis of a radiolabelled chain, it has been shown that the artificial protein is nearly as compact as natural proteins, cooperatively unfolds at high urea concentrations and has some structural features of a definite structure consistent with the designed one. As albebetin has been designed as consisting of two structural repeats, a 'half-albebetin' (one of these repeats) has also been synthesized and studied. It was shown that 'half-albebetin' is also compact.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0269-2139
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1041-52
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:7809031-Amino Acid Sequence,
pubmed-meshheading:7809031-Base Sequence,
pubmed-meshheading:7809031-Chemistry, Physical,
pubmed-meshheading:7809031-Genes, Synthetic,
pubmed-meshheading:7809031-Isotope Labeling,
pubmed-meshheading:7809031-Models, Molecular,
pubmed-meshheading:7809031-Molecular Sequence Data,
pubmed-meshheading:7809031-Peptide Fragments,
pubmed-meshheading:7809031-Physicochemical Phenomena,
pubmed-meshheading:7809031-Protein Biosynthesis,
pubmed-meshheading:7809031-Protein Denaturation,
pubmed-meshheading:7809031-Protein Engineering,
pubmed-meshheading:7809031-Protein Folding,
pubmed-meshheading:7809031-Protein Structure, Secondary,
pubmed-meshheading:7809031-Protein Structure, Tertiary,
pubmed-meshheading:7809031-Proteins,
pubmed-meshheading:7809031-Transcription, Genetic,
pubmed-meshheading:7809031-Urea
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
A new approach to artificial and modified proteins: theory-based design, synthesis in a cell-free system and fast testing of structural properties by radiolabels.
|
| pubmed:affiliation |
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|