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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-2-2
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pubmed:abstractText |
In examinations of the factors regulating the quality and quantity of the immune response to Salmonella typhimurium, we have shown previously that live and heat-killed preparations of S. typhimurium can induce gamma interferon-dominant and interleukin-4-dominant immune responses, respectively, upon intraperitoneal (i.p.) immunization of BALB/c mice. Using this system to investigate the role of the route of immunization in the immune response, we show in the present study that i.p. immunization with heat-killed S. typhimurium generates a quantitatively better immune response than does intradermal (i.d.) immunization. The quantitative differences observed between the i.p. and i.d. routes are apparent in the amount of S. typhimurium-specific antibodies produced, the extent of responses in T-cell proliferation assays, and the quantities of lymphokines generated. However, the ratios of immunoglobulin (Ig) isotypes [IgG1/IgG2a] are comparable and the relative dominance of interleukin-4 over gamma interferon is seen in both i.p.- and i.d.-immunized mice, suggesting that the predominant T-cell effector pathways triggered are not qualitatively dependent on the route of immunization. An examination of the antigenic profile recognised by the B-cell and T-cell responses in i.p.- versus i.d.-immunized mice shows that while the Western immunoblot patterns recognized by serum antibodies from the two groups of mice were not significantly different, T cells from i.p.-immunized mice recognized a broader spectrum of antigens in an immunoblot assay than did those from i.d.-immunized mice. These data suggest that there may be a significant difference in the antigen-processing ability of peritoneal and dermal antigen-presenting cells for complex antigenic formulations such as bacterial vaccines.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-1672641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-1682265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-1683536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-1684784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-1693082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-1824635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-1901883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-2108226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-2121888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-2429917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-2475750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-2903212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-3098864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-3107127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-3281260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-3294331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-7678032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-7903097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7806391-8260457
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
99-103
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7806391-Animals,
pubmed-meshheading:7806391-Antibodies, Bacterial,
pubmed-meshheading:7806391-Antigen-Presenting Cells,
pubmed-meshheading:7806391-B-Lymphocytes,
pubmed-meshheading:7806391-Bacterial Vaccines,
pubmed-meshheading:7806391-Drug Administration Routes,
pubmed-meshheading:7806391-Immunoglobulin Isotypes,
pubmed-meshheading:7806391-Injections, Intradermal,
pubmed-meshheading:7806391-Injections, Intraperitoneal,
pubmed-meshheading:7806391-Interferon-gamma,
pubmed-meshheading:7806391-Interleukin-4,
pubmed-meshheading:7806391-Lymphocyte Activation,
pubmed-meshheading:7806391-Mice,
pubmed-meshheading:7806391-Mice, Inbred BALB C,
pubmed-meshheading:7806391-Salmonella Infections, Animal,
pubmed-meshheading:7806391-Salmonella typhimurium,
pubmed-meshheading:7806391-T-Lymphocytes,
pubmed-meshheading:7806391-Th2 Cells,
pubmed-meshheading:7806391-Vaccines, Inactivated
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pubmed:year |
1995
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pubmed:articleTitle |
Analysis of immunization route-related variation in the immune response to heat-killed Salmonella typhimurium in mice.
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pubmed:affiliation |
National Institute of Immunology, New Delhi, India.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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