rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1995-2-2
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pubmed:databankReference |
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pubmed:abstractText |
A 1670-bp cDNA coding for a novel human cysteine protease has been isolated from a monocyte-derived macrophage cDNA library. This cDNA predicts a 329-amino acid preprocathepsin with more than 50% identity to both human cathepsin S and cathepsin L and 94% identity to a rabbit cDNA, termed OC2, recently isolated from osteoclasts. Based on its high homology to OC2, we have named the human enzyme cathepsin O. Cathepsin O mRNA was identified as a single approximately 1.7 kb transcript in cultures of 15-day-old monocyte-derived macrophages, but was not expressed in human monocytes or alveolar macrophages. When transfected into COS-7 cells, cathepsin O displayed potent endoprotease activity against fibrinogen at acid pH. This novel endoprotease may play an important role in extracellular matrix degradation.
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pubmed:grant |
|
pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0014-5793
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
3
|
pubmed:volume |
357
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
129-34
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7805878-Amino Acid Sequence,
pubmed-meshheading:7805878-Animals,
pubmed-meshheading:7805878-Base Sequence,
pubmed-meshheading:7805878-Cathepsin K,
pubmed-meshheading:7805878-Cathepsins,
pubmed-meshheading:7805878-Cell Line,
pubmed-meshheading:7805878-Cloning, Molecular,
pubmed-meshheading:7805878-Cysteine Endopeptidases,
pubmed-meshheading:7805878-DNA, Complementary,
pubmed-meshheading:7805878-Humans,
pubmed-meshheading:7805878-Macrophages,
pubmed-meshheading:7805878-Molecular Sequence Data,
pubmed-meshheading:7805878-Nucleic Acid Hybridization,
pubmed-meshheading:7805878-Rabbits,
pubmed-meshheading:7805878-Sequence Homology, Amino Acid,
pubmed-meshheading:7805878-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
Molecular cloning of human cathepsin O, a novel endoproteinase and homologue of rabbit OC2.
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pubmed:affiliation |
Physiology Program, Harvard School of Public Health, Boston, MA 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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